An article published this year in “Redox Biology” using one of our products, “PE AnnexinV”, by our customers from the Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM), Madrid, Spain in how Protection against gamma-radiation injury by protein tyrosine phosphatase 1B. Congrats and Thanks.
Summay:
Protein tyrosine phosphatase 1B (PTP1B) is widely expressed in mammalian
tissues, in particular in immune cells, and plays a pleiotropic role in dephosphorylating many
substrates. Moreover, PTP1B expression is enhanced in response to pro-inflammatory stimuli and
to different cell stressors. Taking advantage of the use of mice deficient in
PTP1B we have investigated the effect of γ-radiation in
these animals and found enhanced lethality and decreased respiratory exchange
ratio vs. the
corresponding wild
type animals. Using bone-marrow
derived macrophages and mouse
embryonic fibroblasts (MEFs) from wild-type and PTP1B-deficient mice, we observed a
differential response to various cell stressors. PTP1B-deficient macrophages
exhibited an enhanced response to γ-radiation,
UV-light, LPS and S-nitroso-glutathione. Macrophages exposed to γ-radiation
show DNA
damage and
fragmentation, increased ROS production, a lack in GSH elevation and enhanced
acidic β-galactosidase activity. Interestingly, these differences were not observed in
MEFs. Differential gene expression analysis of WT
and KO macrophages revealed that the main pathways affected after irradiation
were an up-regulation of protein secretion, TGF-β signaling and
angiogenesis among other, and downregulation of Myc targets and Hedgehog signaling. These results demonstrate a key
role for PTP1B in the protection against the cytotoxicity of irradiation in
intact animal and in macrophages, which might be therapeutically relevant.
Reference:
Product link:
PE
AnnexinV
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