An article published this year in “METHODS MOL BIOL” comparing our BASOSTEP
kit with other commercial basophil degranulation kits, by our customers
from Division of Allergy and Immunology, Department of Pediatrics, The Jaffe
Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY,
USA, in the description of the methodology for performing Basophil
Degranulation Assay. Congrats and Thanks.
Summary:
Basophil degranulation assay has
gained importance over the last decade in both diagnosis of food allergy and
evaluation of progression of immunotherapy. This assay involves the
identification and quantification of the expression of CD63 molecule on
basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to
cell membrane during the process of degranulation in which the contents of
basophil granules are released. This chapter describes the methodology for
performing this assay.
CD-sens is defined as the
inverted value for the lowest allergen concentration giving 50% (LC50) of
maximum CD63 upregulation multiplied by 100, i.e., (1/LC50 × 100, where LC50 is
half maximal effective concentration). The higher the value for CD-sens, the
higher is the basophil allergen sensitivity. In addition, change in mean
fluorescent intensities of basophil activation marker CD203 is also useful
readout.
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