An article published this year in “Virus Research” using one of our products, “CF-BlueTM Mouse Anti-Human CD3”, by our customers from the Department of Medical and Clinical Microbiology, Faculty of Medicine, Near East University, Nicosia, Cyprus, in the analysis of The role of Treg population in pathogenesis of Crimean Congo hemorrhagic fever. Congrats and Thanks.
Summay:
Crimean-Congo hemorrhagic fever
(CCHF) is a severe human infection caused by CCHF virus (CCHFV). Today,
although the literature on CCHF pathogenesis is still limited, it is thought to
be associated with immunosuppression in the early phase of infection followed
by pro-inflammatory immune response that may lead to fatal outcomes. The aim of
this study is to investigate the role of regulatory T-cells (Treg cells)
in the pathogenesis of CCHFV.
Peripheral blood mononuclear cell
samples collected from 14 acute CCHF patients with mild disease course and 13
healthy subjects were included in this study. Treg expression
and functional levels were analyzed by flow cytometry. Treg cells
were identified as CD4+CD25 + CD127dim cells, and their functional levels were
compared by measuring their ability to suppress CD69 and CD154 expression by
activated T-cells.
The flow cytometry analysis revealed
that total T-cell and helper T-cell levels did not vary between the two groups.
In contrast, CCHF patients displayed higher Treg cell levels
but lower Treg suppressive activities when compared with
control subjects.
This is the first study on the
involvement of Treg cells in CCHF pathogenesis. Our results
indicate that even though Treg cell levels are elevated during
acute phase of CCHF infection, not all generated Treg cells has
immunosuppressive capacity, and therefore may not represent ‘true’ Treg cell
population. Future studies on the intrinsic mechanisms responsible for the
reduced Treg inhibitory activities are required for further
enlightening the CCHF pathogenesis, especially in the acute phase of the
disease.
Fig. 1. Gating strategy used to sort CD25 + CD127dim Treg cells in CD4 + T-cell population. Following the identification of lymphocytes by forward and size scatter, Treg cells were selected as CD25 + CD127dim cells in the CD3 + CD4 + cell population. The gating strategy resulted in purity of more than %90.
Reference:
Product link:
CF-BlueTM Mouse
Anti-Human CD3
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