An article published in “Nature Comunications” using our some of our mouse antibodies such us Biotin Anti-Mouse CD4 (clone GK1.5), Biotin Anti-Mouse IgM and Biotin Anti-Mouse CD16/32 (clone 2.4G2) by our customers from Spanish National Center of Biotechnology, Madrid, Spain, in the analysis of how Conventional CD4⁺ T cells present bacterial antigens to induce cytotoxic and memory CD8⁺ T cell responses. Congrats and Thanks.

Summary:

Bacterial phagocytosis and antigen cross-presentation to activate CD8⁺T cells are principal functions of professional antigen presenting cells. However, conventional CD4⁺ T cells also capture and kill bacteria from infected dendritic cells in a process termed transphagocytosis (also known as transinfection). Here, we show that transphagocytic T cells present bacterial antigens to naive CD8⁺ T cells, which proliferate and become cytotoxic in response. CD4⁺ T-cell-mediated antigen presentation also occurs in vivo in the course of infection, and induces the generation of central memory CD8⁺ T cells with low PD-1 expression. Moreover, transphagocytic CD4⁺ T cells induce protective anti-tumour immune responses by priming CD8⁺ T cells, highlighting the potential of CD4⁺ T cells as a tool for cancer immunotherapy.

Reference:

https://www.nature.com/articles/s41467-017-01661-7

Products link:

Biotin Anti-Mouse CD4 (clone GK1.5),

http://www.immunostep.com/mouse-antibodies/2927-cd4.html?search_query=MO4B&results=2

Biotin Anti-Mouse CD16/32 (clone 2.4G2)

http://www.immunostep.com/mouse-antibodies/3029-cd16cd32.html?search_query=MO16&results=34

An article published this year in “The University of Valladolid” using our FITC AnnexinV Apoptosis Detection Kit with PI double staining and our FITC mouse anti-human CD3 by our customers from IOBA, Valladolid, Spain, in the analysis of how ALTERATIONS OF THE IMMUNE SYSTEM  OF THE OCULAR MUCOSA IN THE  PEDIATRIC SEVERE OCULAR ALLERGY. Congrats and Thanks.

Summary:

Lymphoid tissue associated with the conjunctiva (CALT) is part of the system immune system of the ocular surface and provide a whole series of defense mechanisms for maintain its integrity. The immunotopographic distribution in healthy individuals can serve to deepen the knowledge of inflammatory pathologies of the ocular surface (OS), like allergic conjunctivitis. This preliminary work aims to initiate the same type of analysis in two serious diseases, of an inflammatory nature, with an allergic base important, atopic keratoconjunctivitis (QCA) and vernal keratoconjunctivitis (QCV). One day today, the diagnosis is clinical, being sometimes difficult the differential diagnosis of these two entities. This study could help to understand some aspects of its pathophysiology.

Reference:

http://uvadoc.uva.es/bitstream/10324/27100/1/TFM-M357.pdf

Products link:

FITC Mouse Anti-Human CD3 Clone 33-2A3:

http://www.immunostep.com/human-antibodies/1691-cd3.html?search_query=CD3+FITC&results=74

FITC AnnexinV Apoptosis Detection Kit with PI double staining:

http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html

An article published this year in the “Journal of Cancer” using one of our products, “FITC Apoptosis Detection Kit”, by our customers from Greece, in the analysis of Potential synergistic effect of phosphodiesterase inhibitors with chemotherapy in lung cancer. Congrats and Thanks.

Summay:

Lung cancer remains the leading cause of cancer-related deaths worldwide and novel therapeutic approaches targeting crucial pathways are urgently needed to improve its treatment. Differentiation-based therapeutics (Methylxanthines) and phosphodiesterase inhibitors (type 4 and 5), have been implicated in cancer treatment. Our objectives were to capture any potential anti-tumor effect of these drug combinations with chemotherapeutic agents in vitro.

Conclusion: The synergistic effect of PDE inhibitors with platinum-based agents has been demonstrated in lung cancer. Our suggestion is that these combinations could be used as additive and maintenance treatment in combination to antineoplastic agents in lung cancer patients.

Reference:

https://www.researchgate.net/profile/Savvas_Petanidis/publication/320466544_Potential_synergistic_effect_of_phosphodiesterase_inhibitors_with_chemotherapy_in_lung_cancer/links/59e715ae0f7e9b13acac6e5e/Potential-synergistic-effect-of-phosphodiesterase-inhibitors-with-chemotherapy-in-lung-cancer.pdf

Product link:

FITC Apoptosis Detection kit with PI

http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html

An article published this year in the “Cellular and Molecular Life Sciences” using one of our Specialized Antibody Services, “Conjugation Service”, by our customers from different centers in Spain and France , in the analysis of Human endoglin as a potential new partner involved in platelet–endothelium interactions. Congrats and Thanks.

Summay:

Complex interactions between platelets and activated endothelium occur during the thrombo-inflammatory reaction at sites of vascular injuries and during vascular hemostasis. The endothelial receptor endoglin is involved in inflammation through integrin-mediated leukocyte adhesion and transmigration; and heterozygous mutations in the endoglin gene cause hereditary hemorrhagic telangiectasia type 1. This vascular disease is characterized by a bleeding tendency that is postulated to be a consequence of telangiectasia fragility rather than a platelet defect, since platelets display normal functions in vitro in this condition. Here, we hypothesize that endoglin may act as an adhesion molecule involved in the interaction between endothelial cells and platelets through integrin recognition. We find that the extracellular domain of human endoglin promotes specific platelet adhesion under static conditions and confers resistance of adherent platelets to detachment upon exposure to flow. Also, platelets adhere to confluent endothelial cells in an endoglin-mediated process. Remarkably, Chinese hamster ovary cells ectopically expressing the human αIIbβ3 integrin acquire the capacity to adhere to myoblast transfectants expressing human endoglin, whereas platelets from Glanzmann’s thrombasthenia patients lacking the αIIbβ3 integrin are defective for endoglin-dependent adhesion to endothelial cells. Furthermore, the bleeding time, but not the prothrombin time, is significantly prolonged in endoglin-haplodeficient (Eng^+/−) mice compared to Eng^+/+ animals. These results suggest a new role for endoglin in αIIbβ3 integrin-mediated adhesion of platelets to the endothelium, and may provide a better understanding on the basic cellular mechanisms involved in hemostasis and thrombo-inflammatory events.

Reference:

https://link.springer.com/article/10.1007/s00018-017-2694-7

Product link:

Specialized Antibody Services

http://www.immunostep.com/content/30-specialized-antibody-services