An article published this year in “MOLECULES” using our AnnexinV-FITC
Apoptosis Detection Kit and propidium iodide (PI)
solution (PI/Rnase), by our customers from Department of Drug Sciences, Medicinal Chemistry
and Pharmaceutical Technology Section, University of Pavia, Italy, in the
analysis of (R)-(−)-Aloesaponol III 8-Methyl Ether from Eremurus persicus, a
Novel Compound against Leishmaniosis. Congrats and Thanks.
Summary:
Leishmaniosis is a neglected
tropical disease which affects several millions of people worldwide. The
current drug therapies are expensive and often lack efficacy, mainly due to the
development of parasite resistance. Hence, there is an urgent need for new
drugs effective against Leishmania infections. As a part of our ongoing study
on the phytochemical characterization and biological investigation of plants
used in the traditional medicine of western and central Asia, in the present
study, we focused on Eremurus persicus root extract in order to evaluate its
potential in the treatment of leishmaniosis. As a result of our study,
aloesaponol III 8-methyl ether (ASME) was isolated for the first time from
Eremurus persicus root extract, its chemical structure elucidated by means of
IR and NMR experiments and the (R) configuration assigned by optical activity
measurements: chiroptical aspects were investigated with vibrational circular
dichroism (VCD) and electronic circular dichroism (ECD) spectroscopies and DFT
(density functional theory) quantum mechanical calculations. Concerning
biological investigations, our results clearly proved that (R)-ASME inhibits
Leishmania infantum promastigotes viability (IC50 73 µg/mL), inducing
morphological alterations and mitochondrial potential deregulation. Moreover,
it is not toxic on macrophages at the concentration tested, thus representing a
promising molecule against Leishmania infections
Reference:
Product link:
FITC Annexin V Apoptosis Detection Kit: http://www.immunostep.com/apoptosis-tools/3727-anxvkf-100t.html
PI/RNASE Solution: http://www.immunostep.com/solutions-buffers-chemicals/1289-pi-rnase-solution.html
Ac2-26 is Annexin A1 (AnxA1) N-terminal-derived peptide, and acts as inhibitor of leukocyte extravasation. It can reduce neutrophil adhesion and emigration, Ac2-26
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