tag:blogger.com,1999:blog-11018829328490177602024-03-05T12:30:08.462-08:00Immunostep NewsletterNewsletterIMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.comBlogger96125tag:blogger.com,1999:blog-1101882932849017760.post-6439605181500269022018-05-29T07:58:00.000-07:002018-05-29T07:58:00.319-07:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.7b00751">Journal of
International Natural Products</a>” </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">FITC Apoptosis
Detection Kit</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext;">the</span><span lang="EN-US" style="background: whitesmoke; color: #737373;"> </span><span lang="EN-US" style="color: windowtext;">CRIOBE,
USR CNRS-EPHE-UPVD, Universitéde Perpignan, France and Universidad de Santiago
de Compostela, Lugo, Spain in how <i style="mso-bidi-font-style: normal;">Structures
and Activities of Tiahuramides A−C, Cyclic Depsipeptides from a Tahitian
Collection of the Marine Cyanobacterium Lyngbya majuscula</i>. Congrats and
Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h2>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">The structures of three new cyclic depsipeptides, tiahuramides A (1), B
(2), and C (3), from a French Polynesian collection of the marine
cyanobacterium Lyngbya majuscula are described. The planar structures of these
compounds were established by a combination of mass spectrometry and 1D and 2D
NMR experiments. Absolute configurations of natural and nonproteinogenic amino
acids were determined through a combination of acid hydrolysis, derivitization
with Marfey’s reagent, and HPLC. The absolute configuration of hydroxy acids
was confirmed by Mosher’s method. The antibacterial activities of tiahuramides
against three marine bacteria were evaluated. Compound 3 was the most active
compound of the series, with an MIC of 6.7 </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">μ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">M on one of
the three tested bacteria. The three peptides inhibit the first cell division
of sea urchin fertilized eggs with IC50 values in the range from 3.9 to 11 </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">μ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">M. Tiahuramide B (2), the most potent compound, causes cellular
alteration characteristics of apoptotic cells, blebbing, DNA condensation, and
fragmentation, already at the first egg cleavage. The cytotoxic activity of
compounds 1−3 was tested in SH-SY5Y human neuroblastoma cells. Compounds 2 and
3 showed an IC50 of 14 and 6.0 </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">μ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">M, respectively,
whereas compound 1 displayed no toxicity in this cell line at 100 </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">μ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">M. To determine the type of cell death induced by tiahuramide C (3),
SH-SY5Y cells were costained with annexin V−FITC and propidium iodide and
analyzed by flow cytometry. The double staining indicated that the cytotoxicity
of compound 3 in this cell line is produced by necrosis.<o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.7b00751">https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.7b00751</a>
<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;">FITC
Apoptosis Detection Kit<o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html</a>
<o:p></o:p></span></b></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-71800824440334271452018-05-29T07:48:00.001-07:002018-05-29T07:48:41.981-07:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="http://www.mdpi.com/1422-0067/19/5/1523/htm">International Journal of
Molecular Sciences</a>” </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">FITC Apoptosis
Detection Kit</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext;">the</span><span lang="EN-US" style="background: whitesmoke; color: #737373;"> </span><span lang="EN-US" style="color: windowtext;">Department of Medicine, Surgery and Health
Sciences, University of Trieste, 34149 Trieste, Italy</span>, in how<span lang="EN-US" style="background: white; color: #222222; font-weight: normal; letter-spacing: 0.15pt;"> </span><span lang="EN-US" style="color: windowtext;">Neuronal
Dysfunction Associated with Cholesterol Deregulatio</span>n. Congrats and
Thanks<span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h2>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="background: white; color: #222222; font-family: "Arial","sans-serif"; font-size: 12.0pt; letter-spacing: .15pt; mso-ansi-language: EN-US;">Cholesterol
metabolism is crucial for cells and, in particular, its biosynthesis in the
central nervous system occurs in situ, and its deregulation involves
morphological changes that cause functional variations and trigger programmed
cell death. The pathogenesis of rare diseases, such as Mevalonate Kinase
Deficiency or Smith–Lemli–Opitz Syndrome, arises due to enzymatic defects in
the cholesterol metabolic pathways, resulting in a shortage of downstream
products. The most severe clinical manifestations of these diseases appear as
neurological defects. Expanding the knowledge of this biological mechanism will
be useful for identifying potential targets and preventing neuronal damage.
Several studies have demonstrated that deregulation of the cholesterol pathway
induces mitochondrial dysfunction as the result of respiratory chain damage. We
set out to determine whether mitochondrial damage may be prevented by using
protective mitochondria-targeted compounds, such as MitoQ, in a neuronal cell
line treated with a statin to induce a biochemical block of the cholesterol
pathway. Evidence from the literature suggests that mitochondria play a crucial
role in the apoptotic mechanism secondary to blocking the cholesterol pathway.
Our study shows that MitoQ, administered as a preventive agent, could counteract
the cell damage induced by statins in the early stages, but its protective role
fades over time.</span><o:p></o:p></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p> </o:p></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small;"><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">Figure. </span><span style="color: #222222; letter-spacing: 0.2px;">Programmed cell death of Lova-treated DAOY cells in the presence of MitoQ. (</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">A</span><span style="color: #222222; letter-spacing: 0.2px;">) Representative cytofluorimetric dot-plots of Annexin propidium iodide staining in all experimental conditions at 12 h (</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">upper</span><span style="color: #222222; letter-spacing: 0.2px;"> panel) and 48 h (</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">lower</span><span style="color: #222222; letter-spacing: 0.2px;"> panel). (</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">B</span><span style="color: #222222; letter-spacing: 0.2px;">,</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">C</span><span style="color: #222222; letter-spacing: 0.2px;">) The results (A/P) obtained as means ± SDs of A+ cells of three independent tests at 12 h (</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">B</span><span style="color: #222222; letter-spacing: 0.2px;">) and 48 h (</span><span style="box-sizing: border-box; color: #222222; font-weight: 700; letter-spacing: 0.2px; line-height: inherit; max-height: 1e+06px;">C</span><span style="color: #222222; letter-spacing: 0.2px;">) (</span><span class="html-italic" style="box-sizing: border-box; color: #222222; font-style: italic; letter-spacing: 0.2px; max-height: 1e+06px;">n</span><span style="color: #222222; letter-spacing: 0.2px;"> = 3, ns: not significant; * </span><span class="html-italic" style="box-sizing: border-box; color: #222222; font-style: italic; letter-spacing: 0.2px; max-height: 1e+06px;">p</span><span style="color: #222222; letter-spacing: 0.2px;"> < 0.05; ** </span><span class="html-italic" style="box-sizing: border-box; color: #222222; font-style: italic; letter-spacing: 0.2px; max-height: 1e+06px;">p</span><span style="color: #222222; letter-spacing: 0.2px;"> < 0.001,*** </span><span class="html-italic" style="box-sizing: border-box; color: #222222; font-style: italic; letter-spacing: 0.2px; max-height: 1e+06px;">p</span><span style="color: #222222; letter-spacing: 0.2px;"> < 0.0001 based on a one-way ANOVA with Bonferroni post-test).</span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://www.mdpi.com/1422-0067/19/5/1523/htm">http://www.mdpi.com/1422-0067/19/5/1523/htm</a><o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;">FITC
Apoptosis Detection Kit<o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html</a>
<o:p></o:p></span></b></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-13269310689677394582018-05-29T07:35:00.002-07:002018-05-29T07:35:36.195-07:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-018-0372-z">Journal
of Nanabiotechnology</a>” </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/human-antibodies/1745-cd9.html?search_query=9PU&results=1">Purified
Mouse Anti-Human CD9</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext;">the</span><span lang="EN-US" style="background: whitesmoke; color: #737373;"> </span><span lang="EN-US" style="color: windowtext;">Universidade da Coruña, DICOMOSA Group, Department
of Psychology, Area of Psychobiology, Edificio de Servicios Centrales de Investigación,
A Coruña, Spain</span>, in how Toxicological assessment of silica-coated iron
oxide nanoparticles in human astrocytes. <span style="mso-spacerun: yes;"> </span><span lang="EN-US" style="color: windowtext;">Congrats
and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h2>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="background: white; color: #333333; font-family: "Noto Serif","serif"; font-size: 13.0pt; mso-ansi-language: EN-US;">Tumour-derived exosomes can be
released to serum and provide information on the features of the malignancy,
however, in order to perform systematic studies in biological samples, faster
diagnostic techniques are needed, especially for detection of low abundance
proteins. Most human cancer cells are positive for at least one ligand for the
activating immune receptor NKG2D and the presence in plasma of NKG2D-ligands
can be associated with prognosis.</span><o:p></o:p></div>
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<span style="font-size: xx-small;"><span style="background-color: white; color: #666666; font-family: "Noto Serif"; text-align: start;">Characterization of exosomes from metastatic melanoma cell lines. Exosomes from Ma-Mel-86c cells were purified from tissue culture supernatant by ultracentrifugation. </span><span class="EmphasisTypeBold" style="background-color: white; box-sizing: inherit; color: #666666; font-family: "Noto Serif"; font-weight: 700; text-align: start;">a</span><span style="background-color: white; color: #666666; font-family: "Noto Serif"; text-align: start;"> TEM. Exosomes were analyzed by electron microscopy. Bar: 100 nm. </span><span class="EmphasisTypeBold" style="background-color: white; box-sizing: inherit; color: #666666; font-family: "Noto Serif"; font-weight: 700; text-align: start;">b</span><span style="background-color: white; color: #666666; font-family: "Noto Serif"; text-align: start;"> Western blot. MICA and tetraspanins (CD63, CD81 and CD9) were analysed by Western blot. Actin was used as loading control. The result from a representative experiment is shown. An exposure of 10–15 s is shown in the right panel. </span><span class="EmphasisTypeBold" style="background-color: white; box-sizing: inherit; color: #666666; font-family: "Noto Serif"; font-weight: 700; text-align: start;">c</span><span style="background-color: white; color: #666666; font-family: "Noto Serif"; text-align: start;"> NKG2D downmodulation. Activated NK cells were co-incubated with increasing amounts of Ma-Mel-86c exosomes as indicated. Cells were stained with anti-NKG2D and analysed by flow cytometry. The plot represents the change in the mean fluorescence intensity (MFI) of NKG2D on NK cells after incubation with exosomes, related to NK cells incubated in medium alone. Data are the mean and SEM obtained in three experiments using NK cells from different donors (*P < 0.05)</span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-018-0372-z">https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-018-0372-z</a><o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;">FITC
Apoptosis Detection Kit<o:p></o:p></span></div>
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<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-70707356736601848092018-05-29T07:20:00.001-07:002018-05-29T07:20:18.454-07:00<h3 style="line-height: 24.0pt; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="https://www.sciencedirect.com/science/article/pii/S0278691518302813#!">Food
and Chemical Toxicology</a>” </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">FITC AnnexinV
Apoptosis Detection Kit</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333;"> </span></span><span lang="EN-US" style="color: windowtext;">the</span><span lang="EN-US" style="background: whitesmoke; color: #737373;"> </span><span lang="EN-US" style="color: windowtext;">Universidade da Coruña, DICOMOSA Group, Department
of Psychology, Area of Psychobiology, Edificio de Servicios Centrales de Investigación,
A Coruña, Spain</span>, in how Toxicological assessment of silica-coated iron
oxide nanoparticles in human astrocytes. <span lang="EN-US" style="color: windowtext;">Congrats
and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h3>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US">Iron oxide </span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/nanoparticles" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Nanoparticles"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">nanoparticles</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"> (ION) have great potential for an increasing number of medical and
biological applications, particularly those focused on </span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/nervous-system" style="box-sizing: border-box; word-break: break-word;" title="Learn more about nervous system"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">nervous system</span></a><span lang="EN-US">. Although ION seem
to be biocompatible and present low toxicity, it is imperative to unveil the
potential risk for the nervous system associated to their exposure, especially
because current data on ION effects on human nervous cells are scarce. Thus, in
the present study potential toxicity associated with silica-coated ION (S-ION)
exposure was evaluated on human A172 glioblastoma cells. To this aim, a
complete toxicological screening testing several exposure times (3 and 24 h),
nanoparticle concentrations (5–100 </span>μ<span lang="EN-US">g/ml), and
culture media (complete and serum-free) was performed to firstly assess S-ION
effects at different levels, including </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/cytotoxicity" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Cytotoxicity"></a><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/cytotoxicity" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Cytotoxicity"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">cytotoxicity</span></a><span lang="EN-US"> – lactate
dehydrogenase assay, analysis of </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/cell-cycle" style="box-sizing: border-box; text-align: start; word-break: break-word;" title="Learn more about Cell Cycle"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">cell cycle</span></a></span><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><span style="box-sizing: border-box; text-align: start;"> and cell death production – and </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/genotoxicity" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Genotoxicity"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">genotoxicity</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"><span style="box-sizing: border-box;"><span style="box-sizing: border-box;"> –
H2AX </span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/phosphorylation" style="box-sizing: border-box; word-break: break-word;" title="Learn more about phosphorylation"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">phosphorylation</span></a><span lang="EN-US"> assessment, </span></span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/comet" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Comet"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">comet</span></a><span lang="EN-US"> assay, micronucleus test and </span></span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/dna" style="box-sizing: border-box; word-break: break-word;" title="Learn more about DNA"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">DNA</span></a><span lang="EN-US"> repair competence assay. Results obtained showed that S-ION
exhibit certain cytotoxicity, especially in serum-free medium, related to cell
cycle disruption and cell death induction. However, scarce genotoxic effects
and no alteration of the DNA repair process were observed. Results obtained in
this work contribute to increase the knowledge on the impact of ION on the
human nervous system cells.</span></span><span style="font-family: Arial, sans-serif; font-size: 12pt;"><o:p></o:p></span></span></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><br /></span></span></div>
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<span lang="EN-US"><span class="label" style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;">Fig</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">. </span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">Transmission electron micrographs of A172 cells incubated with 100 μg/ml of S-ION for 3 h in complete (a) and serum-free (b) medium, and for 24 h in complete (c) medium, showing </span><a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/nanoparticles" style="background-color: transparent; box-sizing: border-box; color: #007398; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start; text-decoration-line: none; word-break: break-word;" title="Learn more about Nanoparticles">nanoparticle</a><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">internalization (arrows indicate S-ION agglomerates). (d) Control A172 cells (not exposed to S-ION). All scale bars (down left side) are 0.5 μm long.</span></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.sciencedirect.com/science/article/pii/S0278691518302813#">https://www.sciencedirect.com/science/article/pii/S0278691518302813#</a>!
<o:p></o:p></span></div>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;">FITC
Apoptosis Detection Kit<o:p></o:p></span></div>
<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-language: AR-SA; mso-fareast-font-family: "Times New Roman"; mso-fareast-language: ES; mso-fareast-theme-font: minor-fareast; mso-no-proof: yes;"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html</a></span>IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-87332248652755449712018-05-29T06:56:00.000-07:002018-05-29T06:56:09.417-07:00<br />
<h2 style="line-height: 24.0pt; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="https://www.sciencedirect.com/science/article/pii/S2213231718301290#!">Redox
Biology</a>” </span><span lang="EN-US" style="color: windowtext;">using one
of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">
</span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/apoptosis/3732-anxvpe-200t.html">PE AnnexinV</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext;">the <span style="mso-spacerun: yes;"> </span>Instituto
de Investigaciones Biomédicas Alberto Sols (CSIC-UAM), Madrid, Spain</span> in how Protection against gamma-radiation injury by
protein tyrosine phosphatase 1B. <span lang="EN-US" style="color: windowtext;">Congrats
and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h2>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ptpn1" title="Learn more about PTPN1"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">Protein tyrosine phosphatase 1B</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><span style="box-sizing: border-box;"> (PTP1B) is widely expressed in mammalian
tissues, in particular in </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/white-blood-cell" style="box-sizing: border-box; word-break: break-word;" title="Learn more about White blood cell"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">immune cells</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><span style="box-sizing: border-box;">, and plays a </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/pleiotropy" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Pleiotropy"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">pleiotropic</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> role in </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/dephosphorylation" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Dephosphorylation"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">dephosphorylating</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> many
substrates. Moreover, </span></span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ptpn1" style="box-sizing: border-box; word-break: break-word;" title="Learn more about PTPN1"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">PTP1B</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> expression is enhanced in response to pro-inflammatory stimuli and
to different cell stressors. Taking advantage of the use of mice deficient in
PTP1B we have investigated the effect of </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">γ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">-radiation in
these animals and found enhanced lethality and decreased respiratory exchange
ratio </span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><span style="font-family: "Arial","sans-serif";"></span>vs.</em></span><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><span style="box-sizing: border-box;"><span style="box-sizing: border-box;"> the
corresponding </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/wild-type" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Wild type"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">wild
type</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> animals. Using bone-marrow
derived </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/macrophages" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Macrophages"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">macrophages</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> and mouse
embryonic </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/fibroblast" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Fibroblast"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">fibroblasts</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> (MEFs) from wild-type and PTP1B-deficient mice, we observed a
differential response to various cell stressors. PTP1B-deficient macrophages
exhibited an enhanced response to </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">γ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">-radiation,
UV-light, LPS and </span></span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/s-nitrosoglutathione" style="box-sizing: border-box; word-break: break-word;" title="Learn more about S-Nitrosoglutathione"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">S-nitroso-glutathione</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">. Macrophages exposed to </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;">γ</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">-radiation
show </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/dna-repair" style="box-sizing: border-box; word-break: break-word;" title="Learn more about DNA repair"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">DNA
damage</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><span style="box-sizing: border-box;"><span style="box-sizing: border-box;"> and
fragmentation, increased ROS production, a lack in GSH elevation and enhanced
acidic </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/beta-galactosidase" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Beta-galactosidase"><span style="color: windowtext;">β</span><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">-galactosidase</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> activity. Interestingly, these differences were not observed in
MEFs. Differential gene </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/expression-analysis" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Expression Analysis"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">expression analysis</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> of WT
and KO macrophages revealed that the main pathways affected after irradiation
were an up-regulation of protein secretion, </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transforming-growth-factor-beta" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Transforming growth factor beta"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">TGF-</span><span style="color: windowtext;">β</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> signaling and
angiogenesis among other, and downregulation of </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/myc" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Myc"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">Myc</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> targets and Hedgehog signaling. These results demonstrate a key
role for PTP1B in the protection against the cytotoxicity of irradiation in
intact animal and in macrophages, which might be therapeutically relevant.</span><o:p></o:p></span></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><b><a href="https://www.sciencedirect.com/science/article/pii/S2213231718301290#">https://www.sciencedirect.com/science/article/pii/S2213231718301290#</a>!
</b><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;">PE
AnnexinV<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/apoptosis/3732-anxvpe-200t.html"><b>http://www.immunostep.com/apoptosis/3732-anxvpe-200t.html</b></a>
<span style="mso-no-proof: yes;"><o:p></o:p></span></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-11519838449476921922018-05-29T05:12:00.000-07:002018-05-29T05:12:07.041-07:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext; line-height: 115%;">An article
published this year in</span><span lang="EN-US" style="color: windowtext; line-height: 115%;"> “<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194789">PlosOne</a>”
</span><span lang="EN-US" style="color: windowtext; line-height: 115%;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">“<a href="http://www.immunostep.com/human-antibodies/1710-cd4.html?search_query=4PP&results=4">PerCP
Mouse anti-Human CD4</a></span><span lang="EN-US" style="line-height: 115%;">”</span><span lang="EN-US" style="color: windowtext; line-height: 115%;">, </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">by our
customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; line-height: 115%; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext; line-height: 115%;">the <span style="mso-spacerun: yes;"> </span>Immunology
Department, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain</span>,
<span style="mso-spacerun: yes;"> </span>In how Levels of anti-CMV antibodies are
modulated by the frequency and intensity of virus reactivations in kidney
transplant patients. <span lang="EN-US" style="color: windowtext; line-height: 115%;">Congrats and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;">.</span></span></h2>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="background: white; color: #333333;"><span style="font-family: Arial, Helvetica, sans-serif;">Anti-CMV (cytomegalovirus) antibody
titers are related to immune alterations and increased risk of mortality. To
test whether they represent a marker of infection history, we analyzed the
effect of viral reactivations on the production of specific antibodies in
kidney transplant patients. We quantified CMV-DNAemia and antibody titers in 58
kidney transplant patients before transplantation and during a follow-up of 315
days (standard deviation, SD: 134.5 days). In order to calculate the intensity
of the infection, we plotted the follow-up time of the infection on the x-axis
and the number of DNA-CMV copies on the y-axis and calculated the area under
the curve (CMV-AUC). The degree of T-lymphocyte differentiation was analyzed
with flow cytometry, the cells were labelled with different monoclonal
antibodies in order to distinguish their differentiation state, from naive
T-cells to senescent T-cells. Peak viremia was significantly higher in patients
experiencing a primary infection (VI) compared to patients experiencing viral
reactivation (VR). Our data indicate that the overall CMV viral load over the
course of a primary infection is significantly higher than in a reactivation of
a previously established infection. Whereas patients who experienced an episode
of CMV reactivation during the course of our observation showed increased
levels of CMV-specific antibodies, patients who did not experience CMV
reactivation (WVR) showed a drop in CMV antibody levels that corresponds to an
overall drop in antibody levels, probably due to the continuing
immunosuppression after the renal transplant. We found a positive correlation
between the CMV viremia over the course of the infection or reactivation and
the CMV-specific antibody titers in the examined patients. We also observed a
positive correlation between anti-CMV titers and T-cell differentiation. In
conclusion, our data show that anti-CMV antibody titers are related to the
course of CMV infection in kidney transplant patients</span></span><span lang="EN-US" style="background: white; color: #333333; font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">.</span><o:p></o:p></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></b></div>
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<span style="font-size: xx-small;"><b><span lang="EN-US" style="color: #333333; font-family: Arial, sans-serif;">Fig. Differentiation status of CD4+ and CD8+ T-lymphocytes according to CMV
serostatus and anti-CMV antibody levels.</span></b><span lang="EN-US" style="font-family: "Times New Roman", serif;"><o:p></o:p></span></span></div>
<br />
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<span lang="EN-US" style="color: #333333; font-family: Arial, sans-serif;"><span style="font-size: xx-small;">(A)
Distribution of CD4+ and CD8+ T-cells into CD28+CD27+ (less-differentiated) and
CD28<sup>null</sup>CD27<sup>null</sup> (more-differentiated) subsets
according to patients CMV serostatus. (B) Distribution of CD4+ and CD8+ T-cells
into CD28+CD27+ (less-differentiated), CD28+CD27<sup>null</sup>/CD28<sup>null</sup>CD27+
(intermediate), and CD28<sup>null</sup>CD27<sup>null</sup> (more-differentiated)
subsets was related to anti-CMV antibody titers in CMV-seropositive patients (n
= 24). Expression of CD28+ and CD27+ was analyzed by flow cytometry in gated
CD3+CD4+ T-cells and in CD3+CD8+ T-cells. Linear regression coefficients
adjusted by age and corresponding p-values are indicated</span><span style="font-size: 10pt;"><o:p></o:p></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194789"><b>http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194789</b></a>
<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">PerCP Mouse anti-Human
CD4<o:p></o:p></span></div>
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<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-53670647733619878092018-05-29T05:04:00.000-07:002018-05-29T05:04:35.407-07:00<br />
<h2 style="line-height: 24.0pt; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="https://www.sciencedirect.com/science/article/pii/S0109564117307327">Dental
Materials</a>” </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/apoptosis/3737-anxvkpe-100t.html">PE Apoptosis Detection
kit</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext;">the</span><span class="MsoHyperlinkFollowed"><span lang="EN-US" style="color: #505050; font-weight: normal;"> </span></span><span lang="EN-US" style="color: windowtext;">Hematopoietic Transplant
and Cellular Therapy Unit, Instituto Murciano de Investigación
Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of
Murcia, Murcia, Spain</span>, <span style="mso-spacerun: yes;"> </span>In how <span lang="EN-US" style="color: windowtext;">Thermo-setting glass ionomer cements promote variable
biological responses of human dental pulp stem cells</span><span class="title-text"><span lang="EN-US" style="color: #505050; font-weight: normal;">. </span></span><span lang="EN-US" style="color: windowtext;">Congrats and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h2>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
<div style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US">Undiluted Equia Forte extracts led to a similar </span><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/cell-growth" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Cell growth"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">cell
proliferation</span></a><span lang="EN-US"> rates than the
control group from 72</span><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> </span><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">h onwards. There were no significance differences between
Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype.
However, in presence of Equia extracts the migration capacity of </span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">h</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">DPSCs was higher
than in presence of Ionostar Molar (</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">p</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> </span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> </span><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">0.05). Also, SEM studies showed a higher degree of cell attachment when
Equia Forte extracts were used. Finally, EDX analysis pointed to different
weight percentages of C, O and Ca ions in glass </span><a href="https://www.sciencedirect.com/topics/materials-science/ionomers" style="box-sizing: border-box; outline-width: 0px; word-break: break-word;" title="Learn more about Ionomers"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">ionomer</span></a><span lang="EN-US"> cements, while other elements such as La, Al, Si, W, Mo and F were
also detected.</span></span></div>
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<span class="label" style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;">Fig. 4</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">. </span><em style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;">h</em><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">DPSCs were cultured on Equia Forte, Ionostar Molar and plastic (control) for 72</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;"> </span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;">h, after which the cells were labelled with Annexin-V and <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/7-aminoactinomycin-d" style="background-color: transparent; box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about 7-Aminoactinomycin D">7-AAD</a><span style="box-sizing: border-box; margin: 0px; padding: 0px;"> and analysed by <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/flow-cytometry" style="background-color: transparent; box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about Flow cytometry">flow cytometry</a>. Numbers within the different quadrants represent the percentages of live (Annexin-V</span></span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">−</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">/7-AAD</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">−</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">), early apoptotic (Annexin-V</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">+</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">/7-AAD</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">−</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">) or late apoptotic and necrotic cells (Annexin-V</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">+</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">/7-AAD</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">+</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;"> and Annexin-V</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">−</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">/7-AAD</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; top: -0.5em; vertical-align: baseline;">+</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">). Dot-plots display representative flow cytometry results obtained after three independent experiments.</span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.sciencedirect.com/science/article/pii/S0109564117307327#">https://www.sciencedirect.com/science/article/pii/S0109564117307327#</a>!<o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">PE Apoptosis
Detection Kit<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-no-proof: yes;"><a href="http://www.immunostep.com/apoptosis/3737-anxvkpe-100t.html">http://www.immunostep.com/apoptosis/3737-anxvkpe-100t.html</a>
<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-27296778274615858532018-05-29T04:53:00.002-07:002018-05-29T04:53:55.583-07:00<h3 style="line-height: 150%; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext; line-height: 150%;">An article published this year in</span><span lang="EN-US" style="color: windowtext; line-height: 150%;"> “<a href="https://www.sciencedirect.com/science/article/pii/S0168170218301175#!">Virus
Research</a>” </span><span lang="EN-US" style="color: windowtext; line-height: 150%;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 150%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 150%;">“<a href="http://www.immunostep.com/human-antibodies/3779-cd3.html?search_query=3CFB&results=2">CF-BlueTM
Mouse Anti-Human CD3</a></span><span lang="EN-US" style="line-height: 150%;">”</span><span lang="EN-US" style="color: windowtext; line-height: 150%;">, </span><span lang="EN-US" style="color: windowtext; line-height: 150%;">by our
customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; line-height: 150%;"> </span></span><span lang="EN-US" style="color: windowtext; line-height: 150%;">the</span><span lang="EN-US" style="color: windowtext; line-height: 150%;"> Department of Medical and Clinical Microbiology,
Faculty of Medicine, Near East University, Nicosia, Cyprus</span>,<span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; line-height: 150%;"> </span></span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 150%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 150%;">in the analysis of The role of Treg population
in pathogenesis of Crimean Congo hemorrhagic fever. </span>Congrats and Thanks<span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 150%;">.</span></span></h3>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Crimean-Congo hemorrhagic fever
(CCHF) is a severe human infection caused by CCHF virus (CCHFV). Today,
although the literature on CCHF pathogenesis is still limited, it is thought to
be associated with immunosuppression in the early phase of infection followed
by pro-inflammatory immune response that may lead to fatal outcomes. The aim of
this study is to investigate the role of regulatory T-cells (T<sub>reg</sub> cells)
in the pathogenesis of CCHFV.<o:p></o:p></span></span></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Peripheral blood mononuclear cell
samples collected from 14 acute CCHF patients with mild disease course and 13
healthy subjects were included in this study. T<sub>reg</sub> expression
and functional levels were analyzed by flow cytometry. T<sub>reg</sub> cells
were identified as CD4+CD25 + CD127dim cells, and their functional levels were
compared by measuring their ability to suppress CD69 and CD154 expression by
activated T-cells.<o:p></o:p></span></span></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">The flow cytometry analysis revealed
that total T-cell and helper T-cell levels did not vary between the two groups.
In contrast, CCHF patients displayed higher T<sub>reg</sub> cell levels
but lower T<sub>reg</sub> suppressive activities when compared with
control subjects.<o:p></o:p></span></span></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">This is the first study on the
involvement of T<sub>reg</sub> cells in CCHF pathogenesis. Our results
indicate that even though T<sub>reg</sub> cell levels are elevated during
acute phase of CCHF infection, not all generated T<sub>reg</sub> cells has
immunosuppressive capacity, and therefore may not represent ‘true’ T<sub>reg</sub> cell
population. Future studies on the intrinsic mechanisms responsible for the
reduced T<sub>reg</sub> inhibitory activities are required for further
enlightening the CCHF pathogenesis, especially in the acute phase of the
disease.</span><span style="font-family: Arial, sans-serif; font-size: 12pt;"><o:p></o:p></span></span></div>
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<span class="label" style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;">Fig. 1</span><span style="color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; text-align: start;">. </span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;">Gating strategy used to sort <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/il-2-receptor" style="box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about IL-2 receptor">CD25 +</a> CD127dim T</span><span style="bottom: -0.25em; box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; vertical-align: baseline;">reg</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;"><span style="box-sizing: border-box; margin: 0px; padding: 0px;"> cells in CD4 + <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/t-cell" style="box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about T cell">T-cell</a> population. Following the identification of </span><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/lymphocyte" style="box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about Lymphocyte">lymphocytes</a> by forward and size scatter, T</span><span style="bottom: -0.25em; box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 9.75px; line-height: 0; margin: 0px; padding: 0px; position: relative; text-align: start; vertical-align: baseline;">reg</span><span style="box-sizing: border-box; color: #737373; font-family: Arial, Helvetica, "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", STIXGeneral, "Cambria Math", "Arial Unicode MS", sans-serif; font-size: 13px; margin: 0px; padding: 0px; text-align: start;"> cells were selected as CD25 + CD127dim cells in the <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/cd3-immunology" style="box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about CD3 (immunology)">CD3</a><span style="box-sizing: border-box; margin: 0px; padding: 0px;"> + <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/cd4" style="box-sizing: border-box; color: #007398; margin: 0px; padding: 0px; text-decoration-line: none; word-break: break-word;" title="Learn more about CD4">CD4 + cell</a> population. The gating strategy resulted in purity of more than %90.</span></span></div>
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<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-49695089369680246282018-05-29T04:40:00.001-07:002018-05-29T04:40:54.691-07:00<br />
<h3 style="background: white; line-height: 12.0pt; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph; vertical-align: baseline;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: large;"><span lang="EN-US" style="color: windowtext;">An article published this
year in</span><span lang="EN-US" style="color: windowtext;"> “<a href="https://www.researchgate.net/profile/Ramon_Garcia-Sanz/publication/319012932_Amiloride_an_old_diuretic_drug_is_a_potential_therapeutic_agent_for_multiple_myeloma/links/59c948c6a6fdcc451d545a92/Amiloride-an-old-diuretic-drug-is-a-potential-therapeutic-agent-for-multiple-myeloma.pdf">Clinical
Cancer Research</a>” </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“<a href="http://www.immunostep.com/apoptosis/3739-kmaf-100t.html">MitoStep + FITC
Apoptosis Detection Kit</a></span><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext;">the Cancer Research Center-IBMCC (USAL-CSIC),
Salamanca, Spain and the Hematology Department, University Hospital of
Salamanca, SpainU,</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; text-decoration: none;"> </span></span><span lang="EN-US" style="color: windowtext; font-weight: normal;"><span style="mso-spacerun: yes;"> </span></span><span lang="EN-US" style="color: windowtext;">in the analysis of how</span><span lang="EN-US"> </span><span lang="EN-US" style="color: windowtext;">Amiloride, an old diuretic drug, is a potential
therapeutic agent for multiple myeloma. </span><span lang="EN-US" style="color: windowtext;">Congrats and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h3>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Amiloride-induced apoptosis was observed in a broad panel of MM cell
lines and in a xenograft mouse model. Moreover, amiloride also had a
synergistic effect when combined with dexamethasone, melphalan, lenalidomide
and pomalidomide. RNA-Seq experiments showed that amiloride not only
significantly altered the level of transcript isoforms and alternative splicing
events, but also deregulated the spliceosomal machinery. Additionally,
disruption of the splicing machinery in immunofluorescence studies was
associated with the inhibition of myeloma cell viability after amiloride
exposure. Although amiloride was able to induce apoptosis in myeloma cells
lacking p53 expression, activation of p53 signaling was observed in wild-type
and mutated TP53 cells after amiloride exposure. On the other hand, we did not
find a significant systemic toxicity in mice treated with amiloride.<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: Arial, sans-serif;"><span style="font-size: xx-small;">Figure. Amiloride induces apoptosis, activates caspases and deregulates
mitochondrial potential in MM cell lines. H929, JJN3 and KMS12-BM cells were treated
with increasing concentrations of amiloride for 24 h. (A) The induction of apoptosis was
Research.
Downloaded from clincancerres.aacrjournals.org on August 9, 2017. © 2017 American Association for Cancer
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited.
Author Manuscript Published OnlineFirst on August 8, 2017; DOI: 10.1158/1078-0432.CCR-17-0678
28
analyzed by flow cytometry after annexin-V/PI staining. (B) Mitochondrial membrane
depolarization was examined by flow cytometry after DilC1(5) staining. (C) The activity of
caspase 8, caspase 9 and caspase 3/7 was analyzed by luminescent caspase assays.
Results are expressed as the mean ± SD of three independent experiments. Statistically
significant differences between untreated and treated cell lines are represented as **p <
0.01 and *p < 0.05 (Student's t-test). </span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.researchgate.net/profile/Ramon_Garcia-Sanz/publication/319012932_Amiloride_an_old_diuretic_drug_is_a_potential_therapeutic_agent_for_multiple_myeloma/links/59c948c6a6fdcc451d545a92/Amiloride-an-old-diuretic-drug-is-a-potential-therapeutic-agent-for-multiple-myeloma.pdf">https://www.researchgate.net/profile/Ramon_Garcia-Sanz/publication/319012932_Amiloride_an_old_diuretic_drug_is_a_potential_therapeutic_agent_for_multiple_myeloma/links/59c948c6a6fdcc451d545a92/Amiloride-an-old-diuretic-drug-is-a-potential-therapeutic-agent-for-multiple-myeloma.pdf</a><o:p></o:p></span></div>
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Apoptosis Detection Kit<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/apoptosis/3739-kmaf-100t.html">http://www.immunostep.com/apoptosis/3739-kmaf-100t.html</a>
<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-21011211500484002372018-05-29T04:19:00.001-07:002018-05-29T04:19:28.102-07:00<br />
<h2 style="line-height: 24.0pt; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article
published this ye</span><span lang="EN-US" style="color: windowtext;">ar in</span><span lang="EN-US" style="color: windowtext;"> “</span><a href="https://www.sciencedirect.com/science/article/pii/S0021979718303631">Journal of Colloid and Interface Science</a>”<span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">using one of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal;"> </span><span lang="EN-US" style="color: windowtext;">“</span><a href="https://www.blogger.com/An%20article%20published%20this%20year%20in%20%E2%80%9CScience%20direct%E2%80%9D%20using%20one%20of%20our%20products,%20%E2%80%9CFITC%20Apoptosis%20Detection%20Kit%E2%80%9D,%20by%20our%20customers%20from%20the%20University%20of%20Zaragoza,%20Spain,%20%20in%20the%20analysis%20of%20how%20Controlled%20release%20of%20bupivacaine%20using%20hybrid%20thermoresponsive%20nanoparticles%20activated%20via%20photothermal%20heating.%20Congrats%20and%20Thanks.%20%20Summay:%20Near-infrared%20(NIR)%20responsive%20nanoparticles%20are%20of%20great%20interest%20in%20the%20biomedical%20field%20as%20antennas%20for%20photothermal%20therapy%20and%20also%20as%20triggers%20for%20on-demand%20drug%20delivery.%20The%20present%20work%20reports%20the%20preparation%20of%20hollow%20gold%20nanoparticles%20(HGNPs)%20with%20plasmonic%20absorption%20in%20the%20NIR%20region%20covalently%20bound%20to%20a%20thermoresponsive%20polymeric%20shell%20that%20can%20be%20used%20as%20an%20on-demand%20drug%20delivery%20system%20for%20the%20release%20of%20analgesic%20drugs.%20The%20photothermal%20heating%20induced%20by%20the%20nanoparticles%20is%20able%20to%20produce%20the%20collapse%20of%20the%20polymeric%20shell%20thus%20generating%20the%20release%20of%20the%20local%20anesthetic%20bupivacaine%20in%20a%20spatiotemporally%20controlled%20way.%20Those%20HGNPs%20contain%20a%2010%E2%80%AFwt.%%20of%20polymer%20and%20present%20excellent%20reversible%20heating%20under%20NIR%20light%20excitation.%20Bupivacaine%20released%20at%20physiological%20temperature%20(37%E2%80%AF%C2%B0C)%20showed%20a%20pseudo-zero%20order%20release%20that%20could%20be%20spatiotemporally%20modified%20on-demand%20after%20applying%20several%20pulses%20of%20light/temperature%20above%20and%20below%20the%20lower%20critical%20solution%20temperature%20(LCST)%20of%20the%20polymeric%20shell.%20Furthermore,%20the%20nanomaterials%20obtained%20did%20not%20displayed%20detrimental%20effects%20on%20four%20mammalian%20cell%20lines%20at%20doses%20up%20to%200.2%E2%80%AFmg/mL.%20From%20the%20results%20obtained%20it%20can%20be%20concluded%20than%20this%20type%20of%20hybrid%20thermoresponsive%20nanoparticle%20can%20be%20used%20as%20an%20externally%20activated%20on-demand%20drug%20delivery%20system.%20%20Reference:%20https://www.sciencedirect.com/science/article/pii/S0021979718303631%20%20Product%20link:%20FITC%20Apoptosis%20Detection%20Kit%20http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html"><span lang="EN-US">FITC Apoptosis Detection Kit</span></a><span lang="EN-US">”</span><span lang="EN-US" style="color: windowtext;">, </span><span lang="EN-US" style="color: windowtext;">by our customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333;"> </span></span><span lang="EN-US" style="color: windowtext;">the University of Zaragoza, Spain,</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333;"> </span></span><span lang="EN-US" style="color: windowtext;">in the analysis of how Controlled
release of bupivacaine using hybrid thermoresponsive nanoparticles activated
via photothermal heating</span><span lang="EN-US" style="color: windowtext;">. Congrats
and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">.</span></span></h2>
<br />
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Near-infrared (NIR) responsive </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemical-engineering/nanoparticles" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Nanoparticles"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">nanoparticles</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> are of great
interest in the biomedical field as antennas for photothermal therapy and also
as triggers for on-demand drug delivery. The present work reports the
preparation of hollow gold nanoparticles (HGNPs) with plasmonic absorption in
the NIR region covalently bound to a thermoresponsive </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/polymer" style="box-sizing: border-box; word-break: break-word;" title="Learn more about polymer"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">polymeric</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> shell that can
be used as an on-demand drug delivery system for the release of </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/antalgic-agent" style="box-sizing: border-box; word-break: break-word;" title="Learn more about antalgic agent"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">analgesic drugs</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">. The photothermal
heating induced by the nanoparticles is able to produce the collapse of the
polymeric shell thus generating the release of the </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/local-anesthetic-agent" style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; widows: 2; word-break: break-word; word-spacing: 0px;" title="Learn more about local anesthetic agent"></a></span><a href="https://www.sciencedirect.com/topics/chemistry/local-anesthetic-agent" style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; widows: 2; word-break: break-word; word-spacing: 0px;" title="Learn more about local anesthetic agent"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">local anesthetic</span></a><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><span style="box-sizing: border-box;"><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><span style="box-sizing: border-box;"> </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/bupivacaine" style="box-sizing: border-box; word-break: break-word;" title="Learn more about bupivacaine"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">bupivacaine</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> in a spatiotemporally controlled way. Those HGNPs contain a
10 wt.% of polymer and present excellent reversible heating under NIR light
excitation. Bupivacaine released at physiological temperature (37 °C) showed a
pseudo-zero order release that could be spatiotemporally modified on-demand
after applying several pulses of light/temperature above and below the lower </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/critical-solution-temperature" style="box-sizing: border-box; word-break: break-word;" title="Learn more about critical solution temperature"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">critical solution temperature</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> (LCST) of the polymeric shell. Furthermore, the </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/nanomaterials" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Nanomaterials"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">nanomaterials</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> obtained did not displayed detrimental effects on four </span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/topics/chemistry/mammalian-cell" style="box-sizing: border-box; word-break: break-word;" title="Learn more about mammalian cell"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">mammalian cell</span></a></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> lines at doses
up to 0.2 mg/mL. From the results obtained it can be concluded than this type
of hybrid thermoresponsive nanoparticle can be used as an externally activated
on-demand drug delivery system.</span><o:p></o:p></span></span></div>
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj6d-tmjrPypm9wfzI5_4VnGr-ML0cglaavA-UBFEW0Msf7sHBZKOIi2OkzDG55889JZgMy2Hwce5mkJx0izXvK3gZwam0czEV7jIaDTcFwvbYzw_xVseQ6nHoIaLtrWzm1F3cGMbXh72k9/s1600/29052018.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" height="177" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEj6d-tmjrPypm9wfzI5_4VnGr-ML0cglaavA-UBFEW0Msf7sHBZKOIi2OkzDG55889JZgMy2Hwce5mkJx0izXvK3gZwam0czEV7jIaDTcFwvbYzw_xVseQ6nHoIaLtrWzm1F3cGMbXh72k9/s400/29052018.jpg" width="400" /></a></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><br /></span></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.sciencedirect.com/science/article/pii/S0021979718303631"><b>https://www.sciencedirect.com/science/article/pii/S0021979718303631</b></a><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">FITC Apoptosis Detection Kit<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html</a>
<o:p></o:p></span></b></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-8866234411980435442018-04-17T09:18:00.003-07:002018-04-17T09:18:46.644-07:00<br />
<h3 style="background: #FCFCFC; margin-bottom: 6.0pt; margin-left: 0cm; margin-right: 0cm; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext; line-height: 115%;">An article
published this year in</span><span lang="EN-US" style="color: windowtext; line-height: 115%;"> “</span><span class="MsoHyperlink"><span lang="EN-US" style="line-height: 115%;"><a href="https://link.springer.com/article/10.1007/s00441-018-2825-y">Springer
Link</a></span></span><span lang="EN-US" style="color: windowtext; line-height: 115%;">”</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">using one
of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">“<a href="http://www.immunostep.com/human-antibodies/2627-cd31.html?search_query=Cd31&results=21">PE
Mouse anti-Human CD31</a></span><span lang="EN-US" style="line-height: 115%;">”</span><span lang="EN-US" style="color: windowtext; line-height: 115%;">, </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">by our
customers from</span><span class="MsoHyperlink"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; line-height: 115%;"> </span></span><span lang="EN-US" style="color: windowtext; line-height: 115%;">Department
of Pharmacology and Razi Drug Research Center, School of Medicine Iran, University
of Medical Sciences Tehran, Iran,</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">in the analysis of how</span><span lang="EN-US" style="color: #333333; font-weight: normal; letter-spacing: 0.1pt;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">Restoring
the IL-1β/NF-κB-induced impaired chondrogenesis by diallyl disulfide in human
adipose-derived mesenchymal stem cells via attenuation of reactive oxygen
species and elevation of antioxidant enzymes</span><span lang="EN-US" style="color: windowtext; line-height: 115%;">. Congrats and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;">.</span></span></h3>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Summay:<o:p></o:p></span></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">Strategies based on
mesenchymal stem cell (MSC) therapy for restoring injured articular cartilage
are not effective enough in osteoarthritis (OA). Due to the enhanced
inflammation and oxidative stress in OA microenvironment, differentiation of
MSCs into chondrocytes would be impaired. This study aims to explore the
effects of diallyl disulfide (DADS) on IL-1</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">β</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">-mediated
inflammation and oxidative stress in human adipose derived mesenchymal stem
cells (hADSCs) during chondrogenesis. MTT assay was employed to examine the
effects of various concentrations of DADS on the viability of hADSCs at
different time scales to obtain non-cytotoxic concentration range of DADS. The
effects of DADS on IL-1</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">β</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">-induced intracellular ROS
generation and lipid peroxidation were evaluated in hADSCs. Western blotting
was used to analyze the protein expression levels of I</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">κ</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">B</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">α</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">
(np), I</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">κ</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">B</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">α</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;"> (p), NF-</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">κ</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">B
(np) and NF-</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">κ</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">B (p). Furthermore, the gene
expression levels of antioxidant enzymes in hADSCs and chondrogenic markers at
days 7, 14 and 21 of differentiation were measured using qRT-PCR. The results
showed that addition of DADS significantly enhanced the mRNA expression levels
of antioxidant enzymes as well as reduced ROS elevation, lipid peroxidation, I</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">κ</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">B</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">α</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">
activation and NF-</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">κ</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">B nuclear translocation in
hADSCs treated with IL-1</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">β</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">. In addition, DADS could
significantly increase the expression levels of IL-1</span><span style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">β</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;">-induced impaired chondrogenic marker genes in
differentiated hADSCs. Treatment with DADS may provide an effective approach to
prevent the pro-inflammatory cytokines and oxidative stress as catabolic causes
of chondrocyte cell death and enhance the protective anabolic effects by
promoting chondrogenesis associated gene expressions in hADSCs exposed to OA
condition.</span><span lang="EN-US" style="background: rgb(252, 252, 252); color: #333333; letter-spacing: 0.1pt;"><o:p></o:p></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Reference:<o:p></o:p></span></span></b></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><a href="https://link.springer.com/article/10.1007/s00441-018-2825-y">https://link.springer.com/article/10.1007/s00441-018-2825-y</a><o:p></o:p></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Product link:<o:p></o:p></span></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="line-height: 115%;"><span style="font-family: Arial, Helvetica, sans-serif;">PE Mouse anti-Human CD31<o:p></o:p></span></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="line-height: 115%;"><span style="font-family: Arial, Helvetica, sans-serif;"><a href="http://www.immunostep.com/human-antibodies/2627-cd31.html?search_query=Cd31&results=21">http://www.immunostep.com/human-antibodies/2627-cd31.html?search_query=Cd31&results=21</a>
<o:p></o:p></span></span></b></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-40955167866836163682018-04-17T09:13:00.000-07:002018-04-17T09:13:03.128-07:00<br />
<h3 style="background: white; margin-top: 0cm;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext; line-height: 115%;">An article published this
year in</span><span lang="EN-US" style="color: windowtext; line-height: 115%;"> “</span><span class="MsoHyperlink"><span lang="EN-US" style="font-weight: normal; line-height: 115%;"><a href="https://search.proquest.com/docview/2013606597/fulltextPDF/C96A64A8424A468CPQ/1?accountid=17252"><b>PlosOne</b></a></span></span><span lang="EN-US" style="color: windowtext; line-height: 115%;">”</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">using one
of our products,</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">“</span><span lang="EN-US" style="line-height: 115%;"><a href="http://www.immunostep.com/solutions-buffers-chemicals/3871-7aad-solution.html?search_query=7AAD&results=1">7AAD</a>”</span><span lang="EN-US" style="color: windowtext; line-height: 115%;">, </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">by our
customers from Department of Internal Medicine, AHEPA University Hospital,
Aristotle University Medical School, Thessaloniki, Greece.</span>,<span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">in the
analysis of how</span><span lang="EN-US" style="color: #777777; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; line-height: 115%;">Prevalence
and correlates of persistent intracellular HIV transcription in individuals on
efavirenz versus atazanavir-based regimens: A prospective cohort study</span><span lang="EN-US" style="color: windowtext; line-height: 115%;">. Congrats and Thanks</span><span lang="EN-US" style="color: windowtext; font-weight: normal; line-height: 115%;">.</span></span></h3>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="mso-ansi-language: EN-US;"><span style="font-family: Arial, Helvetica, sans-serif;">Combination antiretroviral therapy(cART),
albeit not curative, has improved substantially the morbidity and mortality of
HIV disease through prolonged and sustained suppression of viral
replication.[1] The establishment of HIV latency in a population of long lived
memory CD4+ T cells is perceived as the major barrier for the eradication of
HIV infection.[2] Several lines of evidence support the notion of persistent
low level viral replication on cART at least in a subgroup of patients.[3–6]
The importance of persistent HIV transcription(HIVpt) in patients on
suppressive ART is unclear, but several studies suggest that biomarkers of
HIVpt may be promising to assess residual viral replication.[7] There is
limited evidence derived from raltegravir intensification studies that residual
viral replication as evidenced by increases in 2-long terminal repeat circles
may affect to a lesser extent patients on non-nucleoside transcriptase
inhibitors(NNRTIs) versus protease inhibitors (PIs).[3,4] For drugs with steep
dose-response curves such as PIs, small decreases in intracellular
concentrations may diminish significantly the viral inhibition providing a
plausible explanation for this observation.[8] Antiretroviral drugs may be
substrates, inhibitors or inducers of ATP-binding cassette transporters(ABC
transporters) which function as drug efflux pumps with possible implications
for the intracellular concentrations of antiretrovirals.[9] In our prospective
study, we used a method which combines immunophenotyping with
ultrasensitive-FISH to detect unspliced HIV-1 gag-pol in relevant cell
populations in order to compare HIVpt in virologically suppressed patients on
efavirenz(EFV) or atazanavir/ritonavir (ATV/r) and a backbone of
emtricitabine-tenofovir(FTC-TDF).[7,10,11] We followed prospectively our
patient cohort for one year and investigated the impact of HIVpt on virological
outcomes and CD4+ T-cell recovery. Finally, we tested for differences in the
mRNA expression of two widely studied ABC transporters (P-glycoprotein,
P-gp/ABCB1 and multidrug resistance-associated protein-1, MRP1/ABCC1) in
peripheral blood mononuclear cells(PBMCs) between patients with and without
HIVpt by treatment regimen.</span><o:p></o:p></span></div>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://search.proquest.com/docview/2013606597/fulltextPDF/C96A64A8424A468CPQ/1?accountid=17252">https://search.proquest.com/docview/2013606597/fulltextPDF/C96A64A8424A468CPQ/1?accountid=17252</a><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">7AAD<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/solutions-buffers-chemicals/3871-7aad-solution.html?search_query=7AAD&results=1">http://www.immunostep.com/solutions-buffers-chemicals/3871-7aad-solution.html?search_query=7AAD&results=1</a>
<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com1tag:blogger.com,1999:blog-1101882932849017760.post-83553217968855285332018-04-17T08:59:00.003-07:002018-04-17T08:59:48.577-07:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><b><span lang="EN-US">An article published this year in</span></b><span lang="EN-US"> “<span class="MsoHyperlink"><b style="mso-bidi-font-weight: normal;"><a href="https://ieeexplore.ieee.org/abstract/document/8303765/">IEEExplore
Digital Library</a></b></span>”</span><b><span lang="EN-US"> using one of our
products, </span></b><span lang="EN-US">“<b style="mso-bidi-font-weight: normal;"><a href="http://www.immunostep.com/solutions-buffers-chemicals/3872-pi-solution.html?search_query=PI&results=52">Propidium
Iodide</a></b>”, </span><b><span lang="EN-US">by our customers from Department of
Advanced Energy, the University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan.</span>,
in the analysis of how Genotoxicity of tetrahydrofolic acid to hematopoietic
stem and progenitor cells</b><b style="mso-bidi-font-weight: normal;"><span lang="EN-US">. Congrats and Thanks.</span></b></span></h3>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="background: white; color: #333333;"><span style="font-family: Arial, Helvetica, sans-serif;">Plasma irradiation of cancer is
expected to activate a cancer-specific immune response; however, its long-term
systemic effect in animal models has not been sufficiently investigated. In
this study, the long-term systemic effect induced by plasma irradiation was
examined using C57BL/6 mice inoculated with B16F10 melanoma. Melanoma tumors on
the right hind legs of the mice were resected after treatment with nanosecond
pulsed streamer discharge. The results showed that plasma treatment delayed the
growth of recurrent tumors at the site of resection. In addition, plasma
treatment delayed the growth of tumors re-inoculated at the non-irradiated site
on the left leg of the mice after resection. Histological and single-cell
analyses revealed that plasma had no direct effect on primary tumors, whereas
it enhanced cytotoxic T cell infiltration into re-inoculated tumors. These
results suggest that plasma treatment induces cancer-specific long-term immune
memory in mice.</span></span><o:p></o:p></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://ieeexplore.ieee.org/abstract/document/8303765/">https://ieeexplore.ieee.org/abstract/document/8303765/</a><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">Propidium Iodide<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/solutions-buffers-chemicals/3872-pi-solution.html?search_query=PI&results=52">http://www.immunostep.com/solutions-buffers-chemicals/3872-pi-solution.html?search_query=PI&results=52</a><o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-48984247158994931792018-04-17T08:52:00.001-07:002018-04-17T08:52:23.971-07:00IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-60698723329672784822018-04-17T08:42:00.004-07:002018-04-17T08:42:59.062-07:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article published this year in “</span><span lang="EN-US"><a href="https://www.sciencedirect.com/science/article/pii/S0960894X18301811">ScienceDirect</a></span><span lang="EN-US" style="color: windowtext;">” using one of our products, “</span><span class="MsoHyperlink"><a href="http://www.immunostep.com/apoptosis/3731-anxvf-200t.html?search_query=ANXVF&results=1"><span lang="EN-US" style="mso-ansi-language: EN-US;">FITC AnnexinV</span></a></span><span lang="EN-US" style="color: windowtext;">”, by our customers from</span><span lang="EN-US" style="background: whitesmoke; color: #737373;"> </span><span lang="EN-US" style="color: windowtext;">Laboratory of Tumor Molecular Immunobiology, Ludwik
Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland</span>,
in the analysis of how Enantiomeric trans β-aryl-δ-iodo-γ-lactones
derived from 2,5-dimethylbenzaldehyde induce apoptosis in canine lymphoma cell
lines by downregulation of anti-apoptotic Bcl-2 family members Bcl-xL and Bcl-2.
Congrats and Thanks.<br /><b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><o:p> </o:p></span></b></span></h3>
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<o:p></o:p></h1>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US">For many years, studies focused
on developing new natural or synthetic compounds with </span><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/chemotherapy" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Chemotherapy"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">antineoplastic</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"><span style="box-sizing: border-box;"><span style="box-sizing: border-box;"> activity
have attracted the attention of researchers. An interesting group of such
compounds seem to be those with both </span><a href="https://www.sciencedirect.com/topics/chemistry/lactones" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Lactones"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">lactone</span></a><span lang="EN-US"> moiety and an </span></span><a href="https://www.sciencedirect.com/topics/chemistry/aromatic-structure" style="box-sizing: border-box; word-break: break-word;" title="Learn more about aromatic structure"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">aromatic ring</span></a><span lang="EN-US"> which, in
addition to </span></span><a href="https://www.sciencedirect.com/topics/chemistry/antimicrobial-agent" style="box-sizing: border-box; word-break: break-word;" title="Learn more about antimicrobial agent"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">antimicrobial</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"> or </span><a href="https://www.sciencedirect.com/topics/chemistry/antiviral-activity" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Antiviral Activity"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">antiviral activity</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US">, also exhibit antitumor properties. The study shows </span><a href="https://www.sciencedirect.com/topics/chemistry/antitumor-activity" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Antitumor Activity"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">antitumor activity</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"> of two </span><a href="https://www.sciencedirect.com/topics/chemistry/enantiomer" style="box-sizing: border-box; word-break: break-word;" title="Learn more about enantiomer"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">enantiomeric</span></a><span lang="EN-US"> </span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"></em><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">trans</em><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> isomers of
5-(1-iodoethyl)-4-(2′,5′-dimethylphenyl)dihydrofuran-2-one. Our aim was to
determine their antitumor activity manifested as an ability to induce </span><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/apoptosis" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Apoptosis"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">apoptosis</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"> in selected
canine </span><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/cancer-cell-line" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Cancer Cell Line"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">cancer cell lines</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"> as well as to evaluate differences in their strength depending on
the configuration of their stereogenic centers. The </span><a href="https://www.sciencedirect.com/topics/chemistry/enantiomers" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Enantiomers"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">enantiomers</span></a><span lang="EN-US"> (+)-(4</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"></em><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">R</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">,5</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">S</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">,6</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">R</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">)-</span><strong style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">1</strong><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> and (−)-(4</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">S</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">,5</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">R</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">,6</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">S</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">)-</span><strong style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">2</strong><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><span style="box-sizing: border-box;"> were found to induce classical
caspase-dependent apoptosis through downregulation of the expression of </span><a href="https://www.sciencedirect.com/topics/chemistry/antiapoptotic" style="box-sizing: border-box; word-break: break-word;" title="Learn more about antiapoptotic"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">anti-apoptotic</span></a><span lang="EN-US"> proteins </span></span><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/bcl-xl" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Bcl-xL"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">Bcl-xL</span></a><span style="box-sizing: border-box;"></span><span lang="EN-US"> and </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/bcl-2" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Bcl-2"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">Bcl-2</span></a><span lang="EN-US">. Although the mechanism of apoptosis induction was the same for both
enantiomers, they differed in their strength, as stronger antineoplastic
activity </span><em style="box-sizing: border-box;"><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/in-vitro" style="box-sizing: border-box; word-break: break-word;" title="Learn more about In vitro"><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">in vitro</span></span></a></em></span><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> was exhibited by </span><a href="https://www.sciencedirect.com/topics/chemistry/compound-isomer" style="box-sizing: border-box; word-break: break-word;" title="Learn more about compound isomer"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">isomer</span></a><span lang="EN-US"> (+)-(4</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">R</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">,5</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">S</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">,6</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">R</em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">)-</span><strong style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">1</strong><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">.</span></span><span style="font-family: Arial, sans-serif; font-size: 12pt;"> </span></span><span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-no-proof: yes;"><v:shapetype coordsize="21600,21600" filled="f" id="_x0000_t75" o:preferrelative="t" o:spt="75" path="m@4@5l@4@11@9@11@9@5xe" stroked="f">
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<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-11073115446006605592018-04-17T08:32:00.000-07:002018-04-17T08:32:05.746-07:00<br />
<h2 style="background: white; margin-bottom: 10.5pt; margin-left: 0cm; margin-right: 0cm; margin-top: 0cm;">
<span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">An
article published this year in</span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; font-weight: normal; line-height: 115%; mso-ansi-language: EN-US;"> “</span><span lang="EN-US" style="color: #558ed5; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-font-weight: normal; mso-style-textfill-fill-alpha: 100.0%; mso-style-textfill-fill-color: #558ED5; mso-style-textfill-fill-colortransforms: "lumm=60000 lumo=40000"; mso-style-textfill-fill-themecolor: text2; mso-themecolor: text2; mso-themetint: 153;"><a href="https://www.nature.com/articles/s41598-018-21337-6"><span style="color: #558ed5; mso-style-textfill-fill-alpha: 100.0%; mso-style-textfill-fill-color: #558ED5; mso-style-textfill-fill-colortransforms: "lumm=60000 lumo=40000"; mso-style-textfill-fill-themecolor: text2; mso-themecolor: text2; mso-themetint: 153; text-decoration: none; text-underline: none;"><span style="color: windowtext;">Scientific
Reports</span></span></a></span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; font-weight: normal; line-height: 115%; mso-ansi-language: EN-US;">” </span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-font-weight: normal;">u</span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">sing one of our products,</span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; font-weight: normal; line-height: 115%; mso-ansi-language: EN-US;"> “</span><span lang="EN-US" style="color: #558ed5; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-font-weight: normal; mso-style-textfill-fill-alpha: 100.0%; mso-style-textfill-fill-color: #558ED5; mso-style-textfill-fill-colortransforms: "lumm=60000 lumo=40000"; mso-style-textfill-fill-themecolor: text2; mso-themecolor: text2; mso-themetint: 153;"><a href="http://www.immunostep.com/human-antibodies/1662-anti-active-caspase-3.html?search_query=caspase&results=2"><span style="color: #558ed5; mso-style-textfill-fill-alpha: 100.0%; mso-style-textfill-fill-color: #558ED5; mso-style-textfill-fill-colortransforms: "lumm=60000 lumo=40000"; mso-style-textfill-fill-themecolor: text2; mso-themecolor: text2; mso-themetint: 153; text-decoration: none; text-underline: none;"><span style="color: windowtext;">Caspase3</span></span></a></span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; font-weight: normal; line-height: 115%; mso-ansi-language: EN-US;">”,</span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"> by our customers from Department of
Cellular Biology and Immunology, IPBLN-CSIC, Granada, Spain in the analysis of howCD38
promotes pristane-induced chronic inflammation and increases susceptibility to
experimental lupus by an apoptosis-driven and TRPM2-dependent mechanism. </span><span lang="EN-US" style="color: windowtext; font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US; mso-bidi-font-weight: normal;">Congrats
and Thanks.</span></h2>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US" style="background: white; color: #222222; letter-spacing: 0.15pt;">In this study,
we investigated the role of CD38 in a pristane-induced murine model of lupus.
CD38-deficient (</span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Cd38</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">) but not ART2-deficient (</span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Art2</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">) mice developed less severe lupus compared to wild type (WT)
mice, and their protective phenotype consisted of (i) decreased
IFN-I-stimulated gene expression, (ii) decreased numbers of peritoneal CCR2</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">hi</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">hi</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> inflammatory
monocytes, TNF-</span><span style="background: white; color: #222222; letter-spacing: 0.15pt;">α</span><span lang="EN-US" style="background: white; color: #222222; letter-spacing: 0.15pt;">-producing Ly6G</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">+</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> neutrophils and Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">lo</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> monocytes /macrophages, (iii) decreased production of
anti-single-stranded DNA and anti-nRNP autoantibodies, and (iv) ameliorated
glomerulonephritis. </span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Cd38</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> pristane-elicited peritoneal exudate cells had
defective CCL2 and TNF-</span><span style="background: white; color: #222222; letter-spacing: 0.15pt;">α</span><span lang="EN-US" style="background: white; color: #222222; letter-spacing: 0.15pt;"> secretion
following TLR7 stimulation. However, </span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Tnf-</i><i><span style="background: white; color: #222222; letter-spacing: 0.15pt;">α</span></i></span><span lang="EN-US" style="background: white; color: #222222; letter-spacing: 0.15pt;"><span style="font-family: Arial, Helvetica, sans-serif;"><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> and </span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Cxcl12</i><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> gene
expression in </span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Cd38</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> bone marrow (BM) cells was intact, suggesting a
CD38-independent TLR7/TNF-</span><span style="background: white; color: #222222; letter-spacing: 0.15pt;">α</span><span lang="EN-US" style="background: white; color: #222222; letter-spacing: 0.15pt;">/CXCL12 axis in
the BM. Chemotactic responses of </span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Cd38</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">hi</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> monocytes and Ly6G</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">+</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> neutrophils were not impaired. However, </span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Cd38</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">hi</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> monocytes and Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">lo</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">monocytes/macrophages had defective apoptosis-mediated cell
death. Importantly, mice lacking the cation channel TRPM2 (</span><i style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">Trpm2</i><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">−/−</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">) exhibited very
similar protection, with decreased numbers of PECs, and apoptotic Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">hi</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> monocytes and
Ly6C</span><sup style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">lo</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> monocytes/macrophages compared to WT mice. These
findings reveal a new role for CD38 in promoting aberrant inflammation and
lupus-like autoimmunity via an apoptosis-driven mechanism. Furthermore, given
the implications of CD38 in the activation of TRPM2, our data suggest that CD38
modulation of pristane-induced apoptosis is TRPM2-dependent.</span></span><span style="font-family: Arial, sans-serif; font-size: 12pt;"><o:p></o:p></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.nature.com/articles/s41598-018-21337-6">https://www.nature.com/articles/s41598-018-21337-6</a><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">PURE - anti Human
Anti Active Caspase-3<o:p></o:p></span></div>
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<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-2021357565819756942018-02-15T03:53:00.000-08:002018-02-15T03:53:12.453-08:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext;">An article published this year in </span><span class="MsoHyperlink"><span lang="EN-US" style="mso-ansi-language: EN-US;">“</span></span><span class="MsoHyperlink"><span lang="EN-US"><a href="https://www.sciencedirect.com/science/article/pii/S0223523417311248" title="Go to European Journal of Medicinal Chemistry on ScienceDirect">European Journal of Medicinal
Chemistry</a></span></span><span class="MsoHyperlink"><span lang="EN-US" style="mso-ansi-language: EN-US;">”</span></span><span lang="EN-US" style="color: windowtext;"> using some of our products</span><span lang="EN-US" style="color: windowtext; font-weight: normal;">,</span><span lang="EN-US" style="color: windowtext;"> </span><span lang="EN-US" style="color: windowtext; font-weight: normal;">“</span><span lang="EN-US"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">FITC
Apoptosis Detection Kit</a></span></span><span lang="EN-US" style="color: windowtext;"><span style="font-family: Arial, Helvetica, sans-serif; font-size: small;">”
& “<a href="http://www.immunostep.com/solutions-buffers-chemicals/3870-pi-rnase-solution.html?search_query=PI&results=54">PI/RNASE
Solution</a>” by our customers present at the University of Coimbra, Portugal, in
the analysis of how Advances on photodynamic therapy of melanoma through novel
ring-fused 5,15-diphenylchlorins. Congrats and Thanks.</span><span style="font-family: Arial, sans-serif; font-size: 12pt;"><o:p></o:p></span></span></h3>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US">The synthesis, photophysical
behaviour and </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/photosensitizer" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Photosensitizer"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">photosensitization</span></a><span lang="EN-US"> ability
of novel 4,5,6,7-tetrahydropyrazolo[1,5-</span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">a</em><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">]pyridine-fused 5,15-diphenylchlorins against melanoma cells are
described. All studied </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/chlorin" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Chlorin"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">chlorins</span></a><span lang="EN-US"> were found to be extremely active against melanoma cell lines A375
showing IC</span><sub style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">50</sub><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> values below 20 nM. Furthermore, a dihydroxymethyl diphenylchlorin
was identified as an excellent candidate to allow modulating of different types
of </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/apoptosis" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Apoptosis"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">cell
death, apoptosis</span></a><span lang="EN-US"> </span><em style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">vs.</em><span style="-webkit-text-stroke-width: 0px; box-sizing: border-box; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"><span style="box-sizing: border-box;"> necrosis, by varying its
concentration. This can be explored as a tool to improve the effectiveness of
PDT since inflammatory response resulting from necrotic cell death after PDT
can activate the antitumor immune response with implications also regarding the
vascular damage. This feature combined with very low </span><a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/cytotoxicity" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Cytotoxicity"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">cytotoxicity</span></a><span lang="EN-US"> against human melanoma cells in the absence of light activation
and against human </span></span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/fibroblast" style="box-sizing: border-box; word-break: break-word;" title="Learn more about Fibroblast"><span lang="EN-US" style="color: windowtext; mso-ansi-language: EN-US;">fibroblast</span></a><span lang="EN-US"> HFF-1 cells makes this chlorin a candidate of choice as a
photosensitizer for PDT. A comprehensive photophysical investigation including
the determination of quantum yields for fluorescence, singlet oxygen
sensitization and internal conversion, lifetimes and rate constants of all the
excited state deactivation processes has been undertaken.</span></span><b style="mso-bidi-font-weight: normal;"><o:p></o:p></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Reference:<o:p></o:p></span></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><a href="https://www.sciencedirect.com/science/article/pii/S0223523417311248#">https://www.sciencedirect.com/science/article/pii/S0223523417311248#</a>!
<o:p></o:p></span></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US">Product link:</span></b><span lang="EN-US"> <o:p></o:p></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">FITC Apoptosis
Detection Kit</a><o:p></o:p></span></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><a href="http://www.immunostep.com/solutions-buffers-chemicals/3870-pi-rnase-solution.html?search_query=PI&results=54"><span style="font-family: Arial, Helvetica, sans-serif;">PI/RNASE
Solution</span></a><span style="font-family: Arial, sans-serif; font-size: 12pt;"><o:p></o:p></span></span></b></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-36195120486433803232018-02-15T03:42:00.001-08:002018-02-15T03:42:17.315-08:00<br />
<h3 style="background: white; text-align: justify; text-justify: inter-ideograph;">
<span style="font-size: small;"><span lang="EN-US" style="color: windowtext; font-family: Arial, sans-serif; line-height: 115%;">An article published this year in “<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0190307">Plos
One</a>” using some of our products</span><span lang="EN-US" style="color: windowtext; font-family: Arial, sans-serif; font-weight: normal; line-height: 115%;">,</span><span lang="EN-US" style="color: windowtext; font-family: Arial, sans-serif; line-height: 115%;"> </span><span lang="EN-US" style="color: windowtext; font-family: Arial, sans-serif; font-weight: normal; line-height: 115%;">“</span><span lang="EN-US" style="font-family: Arial, sans-serif; line-height: 115%;"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html">FITC Apoptosis
Detection Kit</a></span><span lang="EN-US" style="color: windowtext; font-family: Arial, sans-serif; line-height: 115%;">”,
by our customers present at the Institute of Health Research & Hospital
Lozano Blesa from Zaragoza as well as University and Hospital San Jorge from
Huesca, Spain, in the analysis of Pharmacological activation of TRPV4 produces
immediate cell damage and induction of apoptosis in human melanoma cells and
HaCaT keratinocytes. Congrats and Thanks.</span></span></h3>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="background: white; color: #333333;"><span style="font-family: Arial, Helvetica, sans-serif;">TRPV4
channels are calcium-permeable cation channels that are activated by several
physicochemical stimuli. Accordingly, TRPV4 channels have been implicated in
the regulation of osmosensing, mechanotransduction, thermosensation, and
epithelial/endothelial barrier functions. Whether TRPV4 is also mechanistically
implicated in melanoma cell proliferation is not clear. Here, we hypothesized
that TRPV4 is expressed in human melanoma and that pharmacological activation
interferes with cell proliferation</span></span><span lang="EN-US" style="background: white; color: #333333; font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">.</span><b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p></o:p></span></b></div>
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<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjjKW6QZJ-gm2vuJOAOGDAP6Ke7D9F-4damfuUFGV1LrMrsiCU9CMH_o3lzIBW_HyA01CfSdtkIMj6CZ7HmV6c41j0cURR5pZ0Ey3-cDp9gyxuGzDowh4xgU1zQVqRePM3Siw-X_ir78_u2/s1600/15022018+-+4.PNG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" data-original-height="1436" data-original-width="1600" height="287" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjjKW6QZJ-gm2vuJOAOGDAP6Ke7D9F-4damfuUFGV1LrMrsiCU9CMH_o3lzIBW_HyA01CfSdtkIMj6CZ7HmV6c41j0cURR5pZ0Ey3-cDp9gyxuGzDowh4xgU1zQVqRePM3Siw-X_ir78_u2/s320/15022018+-+4.PNG" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small;">A) Representative flow cytometry dot plots with double Annexin V-FITC/PI staining for control cells (DMSO 0,2%), cells exposed to GSK1016790A (10 nM), and cells exposed to GSK1016790A and HC067047 (1 μM) at 1 h, 24 h, and 72 h. B) Summary data. C) Induction of apoptosis in HaCaT cells and protective effects of HC067047. D) Summary data. *P<0.05 vs. Control, #P<0.05 vs. GSK1016790A, ANOVA, n = 3). Data are means ± SEM (number of independent experiments, n = 3.</span></td></tr>
</tbody></table>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Reference:<o:p></o:p></span></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0190307">http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0190307</a><o:p></o:p></span></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><b style="mso-bidi-font-weight: normal;"><span lang="EN-US">Product link:</span></b><span lang="EN-US"> <o:p></o:p></span></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US"><a href="http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html"><span style="font-family: Arial, Helvetica, sans-serif;">FITC Apoptosis
Detection Kit</span></a></span></b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-40267076634813318112018-02-15T03:08:00.004-08:002018-02-15T03:08:52.439-08:00<br />
<h3 style="background: white; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif;"><b><span lang="EN-US">An article published this year in</span></b><span lang="EN-US"> “<b style="mso-bidi-font-weight: normal;"><a href="https://www.neumosur.net/files/publicaciones/Revistas/2017/3_original2017v29n4.pdf">Revista Española de Patología torácica</a></b>” </span><b><span lang="EN-US">using some </span></b></span><span style="font-family: Arial, Helvetica, sans-serif;"><b><span lang="EN-US">of our products,</span></b><span lang="EN-US"> <b style="mso-bidi-font-weight: normal;">“<a href="http://www.immunostep.com/apoptosis/3734-anxvcfb-200t.html">CF-Blue AnnexinV</a></b>”, </span><b><span lang="EN-US">by our customers present at the IBIS Research </span></b></span><b><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Center, Sevilla, Spain in the analysis of CELULAR ORIGIN OF CIRCULATING </span></span></b><b><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">MICROPARTICLES IN PATIENTS WITH VENOUS THROMBOEMBOLISM AND CANCER. </span></span></b><b><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Congrats and Thanks.</span></span></b></h3>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Micro particles (MPs) are
extracellular vesicles considered to be powerful cellular effectors. They are
present in healthy individuals and are elevated in pathological conditions such
as inflammatory and neoplastic diseases, and thrombosis. The relationship
between venous thromboembolism (VTE) and cancer has been well established. MPs
are thought to be a pathogenic connection between the two entities. If
confirmed, they could be used as biomarkers. Our aim was to characterize the
MPs in both diseases according to their cellular origin (cellular, endothelial,
platelet, leukocyte and those that exhibited mucin 1 on their surface).
Functional parameters such as D-dimer (DD) and soluble P-selectin (sPsel) were
also studied. 96 patients with idiopathic VTE and 85 with advanced lung,
stomach or pancreatic neoplasia were considered. All of them were followed
clinically for two years and those who were diagnosed with cancer in the VTE
group or those who developed thrombosis in the group of neoplastic patients
were excluded from the study. Finally, 82 VTE patients and 68 cancer patients
were analyzed. <o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">In our results, we found that
total MPs and platelet-derived MPs differentiated both patient groups.
Additionally, significantly greater numbers of DD and sPsel <span style="mso-spacerun: yes;"> </span><span style="mso-spacerun: yes;"> </span>(p<0.001)
were determined in the VTE group. The differences found between both groups,
taking into account the origin of the MPs, could be caused by the prothrombotic
characteristics of the neoplastic group and their sequestration within active
clots in the VTE group<b style="mso-bidi-font-weight: normal;"><o:p></o:p></b></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.neumosur.net/files/publicaciones/Revistas/2017/3_original2017v29n4.pdf">https://www.neumosur.net/files/publicaciones/Revistas/2017/3_original2017v29n4.pdf</a>
<o:p></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:</span></b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> <o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/apoptosis/3734-anxvcfb-200t.html">CF-Blue
Apoptosis Detection Kit</a></span></b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-7648671228507391792018-02-15T02:52:00.002-08:002018-02-15T02:52:47.817-08:00<br />
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<span style="font-family: Arial, Helvetica, sans-serif;"><b><span lang="EN-US">An article published
this year in</span></b><span lang="EN-US"> “<b style="mso-bidi-font-weight: normal;"><a href="http://scholar.google.es/scholar_url?url=http://www.mdpi.com/2227-7382/6/1/8/pdf&hl=es&sa=X&scisig=AAGBfm2pu4K7tXJufkSKd6NOKZGQ-_lhOg&nossl=1&oi=scholaralrt">Proteomes</a></b>”
</span><b><span lang="EN-US">using some of our products,</span></b><span lang="EN-US"> <b style="mso-bidi-font-weight: normal;">“<a href="http://www.immunostep.com/human-antibodies/1917-cd14.html?search_query=CD14&results=33">FITC
Mouse Anti-Human CD14</a>, <a href="http://www.immunostep.com/human-antibodies/2571-cd19.html?search_query=CD19&results=100">APC
Mouse Anti-Human CD19</a></b> </span><b><span lang="EN-US">and</span></b><span lang="EN-US"> <a href="http://www.immunostep.com/human-antibodies/2228-cd45.html?search_query=45OC2&results=1"><b style="mso-bidi-font-weight: normal;">OC-515 Mouse Anti-Human CD45</b></a>”, </span><b><span lang="EN-US">by
some of our customers present some Proteomics Units from Universities of Navarra,
Valencia, Madrid, Basque Country and Salamanca, in the analysis of In-Depth
Proteomic Characterization of Classical and Non-Classical Monocyte Subsets Antitumor
Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two
Monocyte Isolation Methods. Congrats and Thanks.</span></b></span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p></o:p></span></div>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">Monocytes are bone marrow-derived
leukocytes that are part of the innate immune system. Monocytes are divided
into three subsets: classical, intermediate and non-classical, which can be
differentiated by their expression of some surface antigens, mainly CD14 and
CD16.<o:p></o:p></span></span></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">These cells are key players in
the inflammation process underlying the mechanism of many diseases. Thus, the
molecular characterization of these cells may provide very useful information for
understanding their biology in health and disease. We performed a multicentric proteomic
study with pure classical and non-classical populations derived from 12 healthy
donors. The robust workflow used provided reproducible results among the five
participating laboratories. Over 5000 proteins were identified, and about half
of them were quantified using a spectral counting approach.<o:p></o:p></span></span></div>
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<span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;">The results represent the protein
abundance catalogue of pure classical and enriched non-classical blood
peripheral monocytes, and could serve as a reference dataset of the healthy
population. The functional analysis of the differences between cell subsets
supports the consensus roles assigned to human monocytes.</span><span style="font-family: Arial, sans-serif; font-size: 12pt;"><o:p></o:p></span></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><br /></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><br /></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg4n6FXIFMRd3W2z53K1WqDjG_ALVlZw5KCkP_BNp77eK5I-Ppbws9j2ljPQmMvIAuLg_dJHwAHkZxmvcfx09xQImvIl-rN27P7zLqBxEoS8vCqUaW6PTJghH397BCJX2r2L6utUVrQZYQq/s1600/15022018-3.png" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" data-original-height="654" data-original-width="712" height="366" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg4n6FXIFMRd3W2z53K1WqDjG_ALVlZw5KCkP_BNp77eK5I-Ppbws9j2ljPQmMvIAuLg_dJHwAHkZxmvcfx09xQImvIl-rN27P7zLqBxEoS8vCqUaW6PTJghH397BCJX2r2L6utUVrQZYQq/s400/15022018-3.png" width="400" /></a></td></tr>
</tbody></table>
<br />
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<br /></div>
<div class="MsoNormal" style="line-height: normal; margin-bottom: 0.0001pt; text-align: justify;">
<span lang="EN-US" style="font-family: URWPalladioL-Roma; font-size: 9.0pt; mso-ansi-language: EN-US; mso-bidi-font-family: URWPalladioL-Roma;">Protein abundance correlation between purified
monocyte populations and public datasets </span><span style="font-family: URWPalladioL-Roma; font-size: 9pt;">obtained from PaxDB. Protein abundances determined in
the present work (y-axis) are plotted against </span><span style="font-family: URWPalladioL-Roma; font-size: 9pt;">public datasets (x-axis). CD14 (classical) and CD16
(non-classical), are shown in left and right panels, </span><span style="font-family: URWPalladioL-Roma; font-size: 9pt;">respectively. Complete monocyte population and liver
datasets are shown in the top panels and bottom </span><span style="font-family: URWPalladioL-Roma; font-size: 9pt;">panels, respectively.</span></div>
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<br /></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><br /></span></div>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
<br />
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://scholar.google.es/scholar_url?url=http://www.mdpi.com/2227-7382/6/1/8/pdf&hl=es&sa=X&scisig=AAGBfm2pu4K7tXJufkSKd6NOKZGQ-_lhOg&nossl=1&oi=scholaralrt">http://scholar.google.es/scholar_url?url=http://www.mdpi.com/2227-7382/6/1/8/pdf&hl=es&sa=X&scisig=AAGBfm2pu4K7tXJufkSKd6NOKZGQ-_lhOg&nossl=1&oi=scholaralrt</a>
<o:p></o:p></span></div>
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<br /></div>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:</span></b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"> <o:p></o:p></span></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/human-antibodies/1917-cd14.html?search_query=CD14&results=33">FITC
Mouse Anti-Human CD14</a><o:p></o:p></span></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/human-antibodies/2571-cd19.html?search_query=CD19&results=100">APC
Mouse Anti-Human CD19</a><o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">
</span></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/human-antibodies/2228-cd45.html?search_query=45OC2&results=1">OC-515
Mouse Anti-Human CD45</a><b><o:p></o:p></b></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-48429260247006776292018-02-15T01:45:00.000-08:002018-02-15T01:45:23.389-08:00<br />
<h2 style="background: white; margin-bottom: 18.0pt; margin-left: 0cm; margin-right: 0cm; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: windowtext; line-height: 115%;">An article published this year in “</span><span class="MsoHyperlink"><span lang="EN-US" style="line-height: 115%;"><a href="https://biologicalproceduresonline.biomedcentral.com/">Biological
Procedures Online</a></span></span><span lang="EN-US" style="color: windowtext; line-height: 115%;">” using one of our products, “</span><span lang="EN-US" style="line-height: 115%;"><a href="http://www.immunostep.com/human-antibodies/1909-hla-dr.html?search_query=HLA-DR&results=18">APC
Mouse anti-Human HLA-DR</a></span><span lang="EN-US" style="color: windowtext; line-height: 115%;">”, by our customers from NOVA Medical School</span>, Faculdade de
Ciências Médicas, Universidade NOVA de Lisboa, Portugal, in the analysis of Antitumor
Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two
Monocyte Isolation Methods. Congrats and Thanks.</span></h2>
<h1 style="background: white; margin-bottom: 18.0pt; margin-left: 0cm; margin-right: 0cm; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<o:p></o:p></h1>
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<br /></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh9ThwZxYaLFCRv-Ue2dK0z3juNWN_QhJ6P75j7SRumQaVoq6Kk4D_EwMULA5tkaepqAhrWGmniANv7a5-Ezm_fq8IMa7lZdsXDtV81gbKD5lL46H1drMU-0OUoeNjU3-Caq4IpBNnfT0g4/s1600/15022018-2.gif" imageanchor="1" style="clear: right; float: right; margin-bottom: 1em; margin-left: 1em;"><img border="0" data-original-height="424" data-original-width="358" height="400" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEh9ThwZxYaLFCRv-Ue2dK0z3juNWN_QhJ6P75j7SRumQaVoq6Kk4D_EwMULA5tkaepqAhrWGmniANv7a5-Ezm_fq8IMa7lZdsXDtV81gbKD5lL46H1drMU-0OUoeNjU3-Caq4IpBNnfT0g4/s400/15022018-2.gif" width="337" /></a><span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US" style="background: white; color: #333333;">Dendritic cells (DCs), which can be
used as anti-cancer vaccines, are generally obtained in vitro from isolated CD14</span><sup style="-webkit-text-stroke-width: 0px; box-sizing: inherit; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;">+</sup><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-align: start; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: 0px;"> monocytes (MoDCs). This generates high cell numbers and
allows instructing DCs to guarantee effective antitumor responses. However, the
impact of the monocyte isolation step in the antitumor effectiveness of the
generated MoDCs is still unknown. Here, we compared the most used
immunomagnetic technologies for monocyte isolation: magnetic activated cell
sorting (MACS) from Miltenyi Biotec and EasySep from STEM CELL.</span></span><o:p></o:p></div>
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<br /></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></b></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></b></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://biologicalproceduresonline.biomedcentral.com/articles/10.1186/s12575-018-0069-6">https://biologicalproceduresonline.biomedcentral.com/articles/10.1186/s12575-018-0069-6</a><o:p></o:p></span></div>
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<br /></div>
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<br /></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><o:p><br /></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
<div class="MsoNormal">
<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/human-antibodies/1909-hla-dr.html?search_query=HLA-DR&results=18">APC
Anti-Human HLA-DR Clone GRB-1</a><o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-2522426681567425372018-02-15T01:31:00.001-08:002018-02-15T03:12:44.886-08:00<br />
<h3 style="background: white; margin: 0cm 0cm 9pt; text-align: justify; vertical-align: baseline;">
<span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;">An article published this year in “</span><span lang="EN-US" style="color: blue; font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;"><a href="https://www.sciencedirect.com/science/article/pii/S0142961218300930">Biomaterials</a></span><span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;">”
using one of our products, “</span><a href="http://www.immunostep.com/apoptosis/3731-anxvf-200t.html"><span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;">FITC ANNEXIN V</span></a><span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;">”, by our customers from the Research
Center Principe Felipe, Valencia, Spain, in the analysis of how Metabolomics
facilitates the discrimination of the specific anti-cancer effects of free- and
polymer-conjugated doxorubicin in breast cancer models. Congrats and Thanks.<o:p></o:p></span></h3>
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<br /></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt;">Summay:<o:p></o:p></span></b></div>
<div class="MsoNormal" style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: "arial" , "helvetica" , sans-serif;"><span lang="EN-US" style="background: white; color: #2e2e2e;">Metabolomics is
becoming a relevant tool for understanding the molecular mechanisms involved in
the response to new drug delivery systems. The applicability of this
experimental approach to cell cultures and animal models makes metabolomics a
useful tool for establishing direct connections between </span><em style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><span style="border: 1pt none windowtext; padding: 0cm;">in vitro</span></em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"> and </span><em style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><span style="border: 1pt none windowtext; padding: 0cm;">in vivo</span></em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"> data,
thus providing a reliable platform for the characterization of chemotherapeutic
agents. Herein, we used metabolomic profiles based on nuclear magnetic
resonance (NMR) spectroscopy to evaluate the biochemical pathways involved in
the response to a chemotherapeutic anthracycline drug (Doxorubicin, Dox) and an
N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer-conjugated form (HPMA-Dox)
in an </span><em style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><span style="border: 1pt none windowtext; padding: 0cm;">in vitro</span></em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"> cell culture model and an </span><em style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><span style="border: 1pt none windowtext; padding: 0cm;">in vivo</span></em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"> orthotopic breast cancer model. We also used
protein expression and flow cytometry studies to obtain a better coverage of
the biochemical alterations associated with the administration of these
compounds. The overall analysis revealed that polymer conjugation leads to
increased apoptosis, reduced glycolysis, and reduced levels of phospholipids
when compared to the free chemotherapeutic drug. Our results represent a first
step in the application of integrated </span><em style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><span style="border: 1pt none windowtext; padding: 0cm;">in vitro</span></em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"> and </span><em style="-webkit-text-stroke-width: 0px; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><span style="border: 1pt none windowtext; padding: 0cm;">in vivo</span></em><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"> metabolomic studies to the evaluation of drug
delivery systems.</span><o:p></o:p></span></div>
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<span style="font-family: "arial" , "helvetica" , sans-serif;"><span style="-webkit-text-stroke-width: 0px; float: none; font-variant-caps: normal; font-variant-ligatures: normal; orphans: 2; text-decoration-color: initial; text-decoration-style: initial; widows: 2; word-spacing: -1.244px;"><br /></span></span></div>
<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEha0uxTVaL1jHA-Egn3TIQyQq0azrzBW4yQ3Or7k0MAow74dHCYX-7hC5LUulEU8LD42iH2N4D1Fh7DkoKBY6t0py_-NdkEwDVwhbSAnQzWaiTuyQx_zdQVsX_nOV3jMGH2omQq8bU0X6Fn/s1600/15022018.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" data-original-height="206" data-original-width="754" height="174" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEha0uxTVaL1jHA-Egn3TIQyQq0azrzBW4yQ3Or7k0MAow74dHCYX-7hC5LUulEU8LD42iH2N4D1Fh7DkoKBY6t0py_-NdkEwDVwhbSAnQzWaiTuyQx_zdQVsX_nOV3jMGH2omQq8bU0X6Fn/s640/15022018.jpg" width="640" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><span class="boldFont" style="background-color: #fcfcfc; border: 0px; color: #5c5c5c; font-family: "arial unicode ms" , "arial unicode" , "arial" , "urw gothic l" , "helvetica" , "tahoma" , "cambria math" , sans-serif; font-size: 10.6667px; font-weight: 700; margin: 0px; padding: 0px; vertical-align: baseline;">(</span><span style="background-color: #fcfcfc; color: #5c5c5c; font-family: "arial unicode ms" , "arial unicode" , "arial" , "urw gothic l" , "helvetica" , "tahoma" , "cambria math" , sans-serif; font-size: 10.6667px;">G), levels of apoptosis after treating MCF7 cells with Dox and HPMA-Dox at 7.5 μg/ml Dox equiv. for 72 h. Cells were stained with AnnexinV and the results are shown as the percentage of positive cells for staining.</span></td></tr>
</tbody></table>
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<span lang="EN-US" style="background: rgb(252 , 252 , 252); color: #333333; letter-spacing: 0.1pt;"><o:p><span style="font-family: "arial" , "helvetica" , sans-serif;"><br /> </span></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt;"><a href="https://www.sciencedirect.com/science/article/pii/S0142961218300930">https://www.sciencedirect.com/science/article/pii/S0142961218300930</a><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;">FITC AnnexinV<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "arial" , "sans-serif"; font-size: 12.0pt; line-height: 115%;"><a href="http://www.immunostep.com/apoptosis/3731-anxvf-200t.html">http://www.immunostep.com/apoptosis/3731-anxvf-200t.html</a>
<o:p></o:p></span></div>
<br />IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-42015709686074032392017-12-29T01:25:00.001-08:002017-12-29T01:25:44.644-08:00<h3 style="background: white; text-align: justify; text-justify: inter-ideograph;">
<span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt;"><span style="font-family: Arial, Helvetica, sans-serif;">An article published in “<a href="https://www.neumosur.net/files/publicaciones/Pendientes-publicar/Remedios-Otero-Candelera-V.pdf">Neumosur.net</a>” using our “<a href="http://www.immunostep.com/apoptosis/3735-anxvkcfb-100t.html?search_query=ANXVKCFB&results=2">CF-Blue Apoptosis Detection Kit</a>” by our </span></span><span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt;"><span style="font-family: Arial, Helvetica, sans-serif;">customers from the Center for Biomedical Research in Respiratory Diseases Network </span></span><span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt;"><span style="font-family: Arial, Helvetica, sans-serif;">(CIBERES), Hospital Universitario Virgen del Rocío de Sevilla, Spain, in the analysis of </span></span><span lang="EN" style="color: #212121;"><span style="font-family: Arial, Helvetica, sans-serif;">CELLULAR ORIGIN OF CIRCULATING MICRO-PARTICLES IN PATIENTS WITH VENOUS </span></span><span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN" style="color: #212121;">THROMBOEMBOLIC DISEASE AND CANCER. </span><span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt;">Congrats and Thanks.</span></span></h3>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summary:<o:p></o:p></span></b></div>
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<span lang="EN" style="color: #212121; font-size: 12pt;"><span style="font-family: Arial, Helvetica, sans-serif;">Microparticles
(MPs) are extracellular vesicles considered to be powerful cellular effectors. They
are present in healthy individuals and are elevated in disease states such as
diseases inflammatory, neoplastic and thrombosis. The relationship between
venous thromboembolic disease (VTE) and cancer is well established It is
thought that MPs would be a pathogenic connection between both entities. If
confirmed, they could be used as biomarkers.<o:p></o:p></span></span></div>
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<span lang="EN" style="color: #212121; font-size: 12pt;"><span style="font-family: Arial, Helvetica, sans-serif;">Our
objective was to characterize the MPs in both pathologies according to their
cellular origin (cellular, endothelial, platelet, leukocyte and those that
exhibited mucin on its surface 1). Functional parameters were also studied such
as dimer D (DD) and soluble P-selectin (sPS).<o:p></o:p></span></span></div>
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<span lang="EN" style="color: #212121; font-size: 12pt;"><span style="font-family: Arial, Helvetica, sans-serif;">We
considered 96 patients with idiopathic VTE and 85 with advanced neoplasms of
lung, gastric or pancreas. To all of them underwent a clinical follow-up of two
years in which those who were excluded from the study were excluded diagnosed
with cancer in the ETV group or who developed thrombosis in the group of neoplastic
patients. Finally, 82 patients with VTE and 68 with cancer were analyzed.<o:p></o:p></span></span></div>
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<span lang="EN" style="color: #212121; font-size: 12pt;"><span style="font-family: Arial, Helvetica, sans-serif;">In
our results we found that the total MPs and the MPs of platelet origin
differentiated both groups of patients. In addition, significantly higher
numbers of DD and sPS were determined (p <0.001) in the group of ETV. The
differences found between both groups, taking into account the origin of the
MPs, could be caused by the prothrombotic characteristics of the neoplastic
group and the sequestration thereof within the clots assets in the ETV group.</span></span><span lang="EN-US" style="color: #212121; font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US; mso-fareast-font-family: "Times New Roman";"><o:p></o:p></span></div>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="color: red; font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.neumosur.net/files/publicaciones/Pendientes-publicar/Remedios-Otero-Candelera-V.pdf">https://www.neumosur.net/files/publicaciones/Pendientes-publicar/Remedios-Otero-Candelera-V.pdf</a>
<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">CF-Blue Apoptosis
Detection Kit<o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/apoptosis/3735-anxvkcfb-100t.html?search_query=ANXVKCFB&results=2">http://www.immunostep.com/apoptosis/3735-anxvkcfb-100t.html?search_query=ANXVKCFB&results=2</a>
<o:p></o:p></span></div>
IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-55189802614864614412017-12-29T01:03:00.000-08:002017-12-29T01:03:30.506-08:00<h3 style="background: white; margin-bottom: 11.25pt; margin-left: 0cm; margin-right: 0cm; margin-top: 0cm; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;"><span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt; line-height: 115%;">An article published in “<a href="http://www.injuryjournal.com/article/S0020-1383(17)30774-X/fulltext">Injury,
International Journal of the Care of the Injured</a>” using our <a href="http://www.immunostep.com/human-antibodies/2692-cd45.html?search_query=CD45+PerCP&results=46">PerCP-Cyanine5.5
Mouse Anti-Human CD45, clone D3/9</a> by our customers from the </span><span lang="EN-US" style="background: rgb(238, 238, 238); color: #333333; line-height: 115%;"> </span><span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt; line-height: 115%;">National Institute of Traumatology and Orthopedics, Rio de Janeiro</span>,
Brazil, in the analysis of how Bone intramedullary reaming grafts the fracture
site with CD146+ skeletal progenitors and downmodulates the inflammatory
environment<span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt; line-height: 115%;">. C</span><span lang="EN-US" style="color: #222222; letter-spacing: 0.15pt; line-height: 115%;">ongrats and Thanks.</span></span></h3>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summary:<o:p></o:p></span></b></div>
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<span style="font-family: Arial, Helvetica, sans-serif;"><span lang="EN-US" style="background: rgb(250, 250, 250); color: #333333;">Femoral shaft
fractures generally occur in young adults following a high-energy trauma and
are prone to delayed union/non-union. Novel therapies to stimulate bone
regeneration will have to mimic some of the aspects of the biology of fracture
healing; however, which are these aspects is unclear. Locked intramedullary
nailing is the current treatment of choice for the stabilisation of femur shaft
fractures, and it is associated with accelerated healing and increased union
rates. These benefits were partially attributed to the reaming procedure,
which, regardless of significantly destroying the haematoma, stimulates the
healing response. To better understand how reaming influences healing, we
evaluated the viability of the nucleated cell fraction and the frequency of
CD146</span><sup>+</sup>skeletal progenitors, which contain multipotent cells, in the
post-reaming haematoma. We also screened the concentrations of inflammatory
mediators and growth factors in the fracture site after reaming compared with
those in the original haematoma.</span><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US; mso-fareast-font-family: "Times New Roman";"><o:p></o:p></span></div>
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<span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 12px; text-align: left;">(A, B) Representative flow cytometry analysis of the CD45</span><span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 9px; line-height: 0; position: relative; text-align: left; top: -0.5em; vertical-align: baseline; white-space: nowrap;">–</span><span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 12px; text-align: left;">/CD31</span><span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 9px; line-height: 0; position: relative; text-align: left; top: -0.5em; vertical-align: baseline; white-space: nowrap;">–</span><span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 12px; text-align: left;">/CD146</span><span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 9px; line-height: 0; position: relative; text-align: left; top: -0.5em; vertical-align: baseline; white-space: nowrap;">+</span><span style="background-color: white; color: #333333; font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; font-size: 12px; text-align: left;"> skeletal cell population in a representative pre- and post-reaming haematoma. Analyses were performed in cells gated subsequently in R1, R2, R3, and R4.</span></div>
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<span lang="EN-US" style="color: red; font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="http://www.injuryjournal.com/article/S0020-1383(17)30774-X/fulltext">http://www.injuryjournal.com/article/S0020-1383(17)30774-X/fulltext</a><o:p></o:p></span></div>
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Mouse Anti-Human CD45, clone D3/9<o:p></o:p></span></div>
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IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0tag:blogger.com,1999:blog-1101882932849017760.post-52295254674018341702017-12-26T01:27:00.000-08:002017-12-26T01:27:52.435-08:00<h2 style="background: white; line-height: normal; margin-bottom: 3.0pt; text-align: justify; text-justify: inter-ideograph;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: large;"><span lang="EN-US" style="color: windowtext;">An article published this year in “</span><span lang="EN-US"><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725127/">Oncotarget</a></span><span lang="EN-US" style="color: windowtext;">” using one of our products, “</span><span lang="EN-US"><a href="http://www.immunostep.com/apoptosis/3737-anxvkpe-100t.html?search_query=ANXVKPE&results=1">PE
Apoptosis Detection Kit</a></span><span lang="EN-US" style="color: windowtext;">”,
by our customers from BioCruces Health Research Institute</span>, Bizkaia,
Spain, in the analysis of how Podocalyxin promotes proliferation and survival
in mature B-cell non-Hodgkin lymphoma cells. Congrats and Thanks.</span><br />
<b><span lang="EN-US"><span style="font-family: Arial, Helvetica, sans-serif;"> </span></span></b></h2>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Summay:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="background-attachment: initial; background-clip: initial; background-image: initial; background-origin: initial; background-position: initial; background-repeat: initial; background-size: initial; font-family: Arial, sans-serif; font-size: 12pt;">Podocalyxin (PCLP1) is a CD34-related
sialomucin expressed by some normal cells and a variety of malignant tumors,
including leukemia, and associated with the most aggressive cancers and poor
clinical outcome. PCLP1 increases breast tumor growth, migration and invasion;
however, its role in hematologic malignancies still remains undetermined. The
purpose of this study was to investigate the expression and function of PCLP1
in mature B-cell lymphoma cells. We found that overexpression of PCLP1
significantly increases proliferation, cell-to-cell interaction, clonogenicity,
and migration of B-cell lymphoma cells. Furthermore, PCLP1 overexpression
results in higher resistance to death induced by dexamethasone, reactive oxygen
species and type II anti-CD20 monoclonal antibody obinutuzumab. Strikingly,
enforced expression of PCLP1 enhances lipid droplet formation as well as
pentose phosphate pathway and glutamine dependence, indicative of metabolic
reprogramming necessary to support the abnormal proliferation rate of tumor
cells. Flow cytometry analysis revealed augmented levels of PCLP1 in malignant
cells from some patients with mature B-cell lymphoma compared to their normal
B-cell counterparts. In summary, our results demonstrate that PCLP1 contributes
to proliferation and survival of mature B-cell lymphoma cells, suggesting that
PCLP1 may promote lymphomagenesis and represents a therapeutic target for the
treatment of B-cell lymphomas.</span><o:p></o:p></div>
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<a href="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi7FspiJuFWLPQ04EQvRM9-NxyX7M8zG6RKfFVxd4xsvmU332w_W2DSQuLAbazr6srmQ6FNfcPTKDsG4ikJPr5EkQ0XpwZg118xM-usGCSIicsDIJBvD6FJwseSJPtI3o5jfo5_2MLRzQk1/s1600/26-12-2017-2.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" data-original-height="795" data-original-width="764" height="640" src="https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi7FspiJuFWLPQ04EQvRM9-NxyX7M8zG6RKfFVxd4xsvmU332w_W2DSQuLAbazr6srmQ6FNfcPTKDsG4ikJPr5EkQ0XpwZg118xM-usGCSIicsDIJBvD6FJwseSJPtI3o5jfo5_2MLRzQk1/s640/26-12-2017-2.jpg" width="609" /></a></div>
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<strong style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">A</strong><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">) Raji-Ctrl and Raji-PCLP1 cells were treated with increasing concentrations of vehicle or dexamethasone for 72 hours and cell death was analyzed after Annexin V-PE/7´AAD staining by flow cytometry. The graph on the left shows the mean percentages ± SD of total specific cell death (including early apoptosis, late apoptosis and necrotic cells) from six independent experiments after subtracting the spontaneous cell death (vehicle). The percentage of specific cell death was calculated as follows: [(% lysis of target cell – % spontaneous cell death)/(100% – % spontaneous cell death)] × 100. Representative dot plots showing Annexin V/7AAD staining in cells treated with vehicle or 1000 µM dexamethasone are shown (right). As displayed in the dot plots, the spontaneous cell death (Annexin V PE positive/7ADD negative, Annexin V PE positive/7ADD positive and Annexin V PE negative/7ADD positive cells) in cells cultured with the vehicle was approximately 7.5% in Raji-Ctrl and 5.5% in Raji-PCLP1 cells. (</span><strong style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">B</strong><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">) Expression of PCLP1 on Annexin V and 7ADD double-negative population of cells treated with dexamethasone or vehicle by flow cytometry. (</span><strong style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">C</strong><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">) Raji-Ctrl and Raji-PCLP1 cells were treated with increasing concentrations of vehicle or H</span><span style="bottom: -0.25em; font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: 0.25em; vertical-align: baseline;">2</span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">O</span><span style="bottom: -0.25em; font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: 0.25em; vertical-align: baseline;">2</span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;"> for 6 h and cell death was analyzed as indicated in A. The graph on the left shows the mean percentages ± SD of total cell death from five independent experiments after subtracting the spontaneous cell death (vehicle). Representative dot plots showing Annexin V/7AAD staining in cells treated with vehicle or 200 µM H</span><span style="bottom: -0.25em; font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: 0.25em; vertical-align: baseline;">2</span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">O</span><span style="bottom: -0.25em; font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: 0.25em; vertical-align: baseline;">2</span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;"> are shown (left). As displayed in the dot plots, the spontaneous cell death in the presence of vehicle was approximately 17.0% in Raji-Ctrl and 5.9% in Raji-PCLP1 cells. (</span><strong style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">D</strong><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">) Cells were incubated overnight with increasing concentrations of obinutuzumab or vehicle and cell viability was measured based on Annexin V/7AAD double negative staining relative to vehicle-treated cells. Data show the mean percentages ± SD of viable cells from six independent experiments. (</span><strong style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">E</strong><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">) A representative image of the homotypic adhesion induced by obinutuzumab as visualized by light microscopy is shown. The size bar indicates 100 µm. Statistical analysis was calculated using two-tailed paired Student´s </span><em style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">t</em><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;">-test. </span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: -0.5em; vertical-align: baseline;">*</span><em style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">P</em><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;"> < 0.05. </span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: -0.5em; vertical-align: baseline;">**</span><em style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">P</em><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;"> < 0.01. </span><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; line-height: 1.6363em; position: relative; text-align: left; top: -0.5em; vertical-align: baseline;">***</span><em style="font-family: Arial, Helvetica, sans-serif; font-size: x-small; text-align: left;">P</em><span style="font-family: Arial, Helvetica, sans-serif; font-size: xx-small; text-align: left;"> < 0.001.</span></div>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Reference:</span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;"><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725127/">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725127/</a><o:p></o:p></span></div>
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<b><span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-ansi-language: EN-US;">Product link:<o:p></o:p></span></b></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;">PE Apoptosis
Detection Kit <o:p></o:p></span></div>
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<span lang="EN-US" style="font-family: "Arial","sans-serif"; font-size: 12.0pt; line-height: 115%; mso-ansi-language: EN-US;"><a href="http://www.immunostep.com/apoptosis/3737-anxvkpe-100t.html?search_query=ANXVKPE&results=1">http://www.immunostep.com/apoptosis/3737-anxvkpe-100t.html?search_query=ANXVKPE&results=1</a><o:p></o:p></span></div>
IMMUNOSTEPhttp://www.blogger.com/profile/03514072061131740939noreply@blogger.com0