An article published this year in “NUTRIENTS” using our Annexin
V-FITC Apoptosis Detection Kit, by our customers from Department of
Paediatrics, Institute for Maternal and Child Health, IRCCS Burlo Garofolo,
Trieste, Italy, in the analysis of Curcumin Anti-Apoptotic Action in a Model of
Intestinal Epithelial Inflammatory Damage. Congrats and Thanks.
Summary:
The purpose of this study is to
determine if a preventive treatment with curcumin can protect intestinal
epithelial cells from inflammatory damage induced by IFNγ. To achieve this goal
we have used a human intestinal epithelial cell line (HT29) treated with IFNγ
to undergo apoptotic changes that can reproduce the damage of intestinal
epithelia exposed to inflammatory cytokines. In this model, we measured the
effect of curcumin (curcuminoid from Curcuma Longa) added as a pre-treatment at
different time intervals before stimulation with IFNγ. Curcumin administration
to HT29 culture before the inflammatory stimulus IFNγ reduced the cell
apoptosis rate. This effect gradually declined with the reduction of the
curcumin pre-incubation time. This anti-apoptotic action by curcumin
pre-treatment was paralleled by a reduction of secreted IL7 in the HT29 culture
media, while there was no relevant change in the other cytokine levels. Even
though curcumin pre-administration did not impact the activation of the NF-κB
pathway, a slight effect on the phosphorylation of proteins in this
inflammatory signaling pathway was observed. In conclusion, curcumin
pre-treatment can protect intestinal cells from inflammatory damage. These
results can be the basis for studying the preventive role of curcumin in
inflammatory bowel disease
Reference:
Product link:
FITC Annexin V Apoptosis Detection Kit with PI: http://www.immunostep.com/apoptosis-tools/3727-anxvkf-100t.html
No comments:
Post a Comment