An article published this year in “International Journal of Molecular Sciences” using one of our products, “FITC Apoptosis Detection Kit”, by our customers from the Department of Medicine, Surgery and Health Sciences, University of Trieste, 34149 Trieste, Italy, in how Neuronal Dysfunction Associated with Cholesterol Deregulation. Congrats and Thanks.
Summay:
Cholesterol
metabolism is crucial for cells and, in particular, its biosynthesis in the
central nervous system occurs in situ, and its deregulation involves
morphological changes that cause functional variations and trigger programmed
cell death. The pathogenesis of rare diseases, such as Mevalonate Kinase
Deficiency or Smith–Lemli–Opitz Syndrome, arises due to enzymatic defects in
the cholesterol metabolic pathways, resulting in a shortage of downstream
products. The most severe clinical manifestations of these diseases appear as
neurological defects. Expanding the knowledge of this biological mechanism will
be useful for identifying potential targets and preventing neuronal damage.
Several studies have demonstrated that deregulation of the cholesterol pathway
induces mitochondrial dysfunction as the result of respiratory chain damage. We
set out to determine whether mitochondrial damage may be prevented by using
protective mitochondria-targeted compounds, such as MitoQ, in a neuronal cell
line treated with a statin to induce a biochemical block of the cholesterol
pathway. Evidence from the literature suggests that mitochondria play a crucial
role in the apoptotic mechanism secondary to blocking the cholesterol pathway.
Our study shows that MitoQ, administered as a preventive agent, could counteract
the cell damage induced by statins in the early stages, but its protective role
fades over time.
Figure. Programmed cell death of Lova-treated DAOY cells in the presence of MitoQ. (A) Representative cytofluorimetric dot-plots of Annexin propidium iodide staining in all experimental conditions at 12 h (upper panel) and 48 h (lower panel). (B,C) The results (A/P) obtained as means ± SDs of A+ cells of three independent tests at 12 h (B) and 48 h (C) (n = 3, ns: not significant; * p < 0.05; ** p < 0.001,*** p < 0.0001 based on a one-way ANOVA with Bonferroni post-test).
Reference:
Product link:
FITC
Apoptosis Detection Kit
No comments:
Post a Comment