An article published this year in “Dental Materials” using one of our products, “PE Apoptosis Detection kit”, by our customers from the Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain, In how Thermo-setting glass ionomer cements promote variable biological responses of human dental pulp stem cells. Congrats and Thanks.
Summay:
Undiluted Equia Forte extracts led to a similar cell
proliferation rates than the
control group from 72 h onwards. There were no significance differences between
Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype.
However, in presence of Equia extracts the migration capacity of hDPSCs was higher
than in presence of Ionostar Molar (p < 0.05). Also, SEM studies showed a higher degree of cell attachment when
Equia Forte extracts were used. Finally, EDX analysis pointed to different
weight percentages of C, O and Ca ions in glass ionomer cements, while other elements such as La, Al, Si, W, Mo and F were
also detected.
Fig. 4. hDPSCs were cultured on Equia Forte, Ionostar Molar and plastic (control) for 72 h, after which the cells were labelled with Annexin-V and 7-AAD and analysed by flow cytometry. Numbers within the different quadrants represent the percentages of live (Annexin-V−/7-AAD−), early apoptotic (Annexin-V+/7-AAD−) or late apoptotic and necrotic cells (Annexin-V+/7-AAD+ and Annexin-V−/7-AAD+). Dot-plots display representative flow cytometry results obtained after three independent experiments.
Reference:
Product link:
PE Apoptosis
Detection Kit
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