An article published this year in “Food and Chemical Toxicology” using one of our products, “FITC AnnexinV Apoptosis Detection Kit”, by our customers from the Universidade da Coruña, DICOMOSA Group, Department of Psychology, Area of Psychobiology, Edificio de Servicios Centrales de Investigación, A Coruña, Spain, in how Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes. Congrats and Thanks.
Summay:
Iron oxide nanoparticles (ION) have great potential for an increasing number of medical and
biological applications, particularly those focused on nervous system. Although ION seem
to be biocompatible and present low toxicity, it is imperative to unveil the
potential risk for the nervous system associated to their exposure, especially
because current data on ION effects on human nervous cells are scarce. Thus, in
the present study potential toxicity associated with silica-coated ION (S-ION)
exposure was evaluated on human A172 glioblastoma cells. To this aim, a
complete toxicological screening testing several exposure times (3 and 24 h),
nanoparticle concentrations (5–100 μg/ml), and
culture media (complete and serum-free) was performed to firstly assess S-ION
effects at different levels, including cytotoxicity – lactate
dehydrogenase assay, analysis of cell cycle and cell death production – and genotoxicity –
H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION
exhibit certain cytotoxicity, especially in serum-free medium, related to cell
cycle disruption and cell death induction. However, scarce genotoxic effects
and no alteration of the DNA repair process were observed. Results obtained in
this work contribute to increase the knowledge on the impact of ION on the
human nervous system cells.
Fig. Transmission electron micrographs of A172 cells incubated with 100 μg/ml of S-ION for 3 h in complete (a) and serum-free (b) medium, and for 24 h in complete (c) medium, showing nanoparticleinternalization (arrows indicate S-ION agglomerates). (d) Control A172 cells (not exposed to S-ION). All scale bars (down left side) are 0.5 μm long.
Reference:
Product link:
FITC
Apoptosis Detection Kit
http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html
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