An article published this year in “Journal of Nanabiotechnology” using one of our products, “Purified Mouse Anti-Human CD9”, by our customers from the Universidade da Coruña, DICOMOSA Group, Department of Psychology, Area of Psychobiology, Edificio de Servicios Centrales de Investigación, A Coruña, Spain, in how Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes. Congrats and Thanks.
Summay:
Tumour-derived exosomes can be
released to serum and provide information on the features of the malignancy,
however, in order to perform systematic studies in biological samples, faster
diagnostic techniques are needed, especially for detection of low abundance
proteins. Most human cancer cells are positive for at least one ligand for the
activating immune receptor NKG2D and the presence in plasma of NKG2D-ligands
can be associated with prognosis.
Characterization of exosomes from metastatic melanoma cell lines. Exosomes from Ma-Mel-86c cells were purified from tissue culture supernatant by ultracentrifugation. a TEM. Exosomes were analyzed by electron microscopy. Bar: 100 nm. b Western blot. MICA and tetraspanins (CD63, CD81 and CD9) were analysed by Western blot. Actin was used as loading control. The result from a representative experiment is shown. An exposure of 10–15 s is shown in the right panel. c NKG2D downmodulation. Activated NK cells were co-incubated with increasing amounts of Ma-Mel-86c exosomes as indicated. Cells were stained with anti-NKG2D and analysed by flow cytometry. The plot represents the change in the mean fluorescence intensity (MFI) of NKG2D on NK cells after incubation with exosomes, related to NK cells incubated in medium alone. Data are the mean and SEM obtained in three experiments using NK cells from different donors (*P < 0.05)
Reference:
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FITC
Apoptosis Detection Kit
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