An article published this year in “JOURNAL OF CELLULAR PHYSIOLOGY” using
our anti Human
CD105 (Clon 2H6F11), by our customers from Dipartimento Scienze Cliniche e
Molecolari, Clinica di Ematologia, Università Politecnica delle Marche, Ancona,
Italy, in the analysis of how Bone marrow adipocytes support haematopoietic
stem cell survival. Congrats and Thanks.
Summary:
In bone marrow (BM), haematopoietic
elements are mingled with adipocytes (BM-A), which are the most abundant
stromal component in the niche. BM-A progressively increase with ageing,
eventually occupying up to 50% of BM cavities.
In this work, the role played by
BM-A was explored by studying primary human BM-A isolated from hip surgery
patients at the molecular level, through microarray analysis, and at the
functional level, by assessing their relationship with primary human
haematopoietic stem cells (HSC) by the long-term culture initiating cell
(LTC-IC) assay.
Findings demonstrated that BM-A are
capable of supporting HSC survival in the LTC-IC assay, since after 5 weeks of
co-culture, HSC were still able to proliferate and differentiate. Furthermore,
critical molecules such as C-X-C motif chemokine 12 (CXCL12), interleukin
(IL)-8, colony-stimulating factor 3 (CSF3), and leukaemia inhibitory factor
(LIF), were expressed at similar levels in BM-A and in primary human BM
mesenchymal stromal cells (BM-MSC), whereas IL-3 was higher in BM-A.
Interestingly, BM-A displayed a
different gene expression profile compared with subcutaneous adipose tissue
adipocytes (AT-A) collected from abdominal surgery patients, especially in
terms of regulation of lipid metabolism, stemness genes and white-to-brown
differentiation pathways. Accordingly, analysis of the gene pathways involved
in haematopoiesis regulation showed that BM-A are more closely related to
BM-MSC than to AT-A.
The present data suggest that BM-A
play a supporting role in the haematopoietic niche and directly sustain HSC
survival.
Reference:
Product link:
Anti Human CD105 (clon 2H6F1): http://www.immunostep.com/antibodies/2792-cd105.html
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