An article published this year in “NANOSCALE” using our CFBlue Annexin V
Apoptosis Detection Kit with PI, by our customers from Department of Medicine and
General Cytometry Service-Nucleus, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL),
Salamanca, Spain, in the analysis of Functional Insights into the Cellular
Response Triggered by Bile-Acid Platinum Compound Conjugated to Biocompatible
Ferric Nanoparticles Using Quantitative Proteomic Approaches. Congrats and
Thanks.
Summary:
At present, bioferrofluids are
employed as multifunctional powerful tools for biomedical applications such as
drug delivery, among others. The present study explores the cellular response
when bile-acid platinum derivatives are conjugated with bioferrofluids by
testing the biological activity in osteosarcoma (MG-63) and T-cell leukemia
(Jurkat) cells. The aim of this work is to evaluate the biocompatibility of a
bile-acid platinum derivative conjugated with multi-functional polymer coated
bioferrofluids by observing the effects on the protein expression profiles and
in intracellular pathways of the nanoparticle-stimulated cells. To this end, a
mass spectrometry-based approach termed SILAC has been applied to determine in
a high-throughput manner the key proteins involved in the cellular respond
process (including specific quantitatively identified proteins related to the
vesicular transport, cellular structure, cell cycle, biosynthetic process,
apoptosis and regulation of the cell cycle). Finally, the biocompatibility was
evaluated and validated also by conventional strategies (such as flow cytometry
and MTT)
Reference:
Product link:
CFBlue Annexin V Apoptosis Detection Kit with PI: http://www.immunostep.com/apoptosis-tools/3735-anxvkcfb-100t.html
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