An article published this year in “European Journal of Medicinal
Chemistry” using some of our products, “FITC
Apoptosis Detection Kit”
& “PI/RNASE
Solution” by our customers present at the University of Coimbra, Portugal, in
the analysis of how Advances on photodynamic therapy of melanoma through novel
ring-fused 5,15-diphenylchlorins. Congrats and Thanks.
Summay:
The synthesis, photophysical
behaviour and photosensitization ability
of novel 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridine-fused 5,15-diphenylchlorins against melanoma cells are
described. All studied chlorins were found to be extremely active against melanoma cell lines A375
showing IC50 values below 20 nM. Furthermore, a dihydroxymethyl diphenylchlorin
was identified as an excellent candidate to allow modulating of different types
of cell
death, apoptosis vs. necrosis, by varying its
concentration. This can be explored as a tool to improve the effectiveness of
PDT since inflammatory response resulting from necrotic cell death after PDT
can activate the antitumor immune response with implications also regarding the
vascular damage. This feature combined with very low cytotoxicity against human melanoma cells in the absence of light activation
and against human fibroblast HFF-1 cells makes this chlorin a candidate of choice as a
photosensitizer for PDT. A comprehensive photophysical investigation including
the determination of quantum yields for fluorescence, singlet oxygen
sensitization and internal conversion, lifetimes and rate constants of all the
excited state deactivation processes has been undertaken.
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