An article published this year in “THE JOURNAL OF IMMUNOLOGY” using our Specialized Antibody
Services
for Anti– TNF-a directly labeling with CF-Blue, by our customers from
University Pompeu Fabra,, Hospital del Mar Medical Research Institute and Department
of Immunology, Hospital del Mar, Barcelona, Spain, in the analysis of how
Antibody-Dependent NK Cell Activation Differentially Targets EBV-Infected Cells
in Lytic Cycle and Bystander B Lymphocytes Bound to Viral Antigen–Containing
Particles. Congrats and Thanks.
Summary:
NK cells have been reported to
respond against EBV-infected B cells in the lytic cycle and to control the
viral infection involving IFN-γ secretion. Early reports proposed a role for NK
cell Ab-dependent cellular cytotoxicity (ADCC) triggered via FcγR-IIIA (CD16)
in the response to EBV. In the current study, we revisited this issue, showing
that serum from EBV+ individuals triggered vigorous NK cell degranulation and
cytokine production (i.e., TNF-α and IFN-γ) against EBV-infected cells,
enhancing NK cell activation. The effect was preferentially directed against
cells in the lytic phase and was associated with surface expression of the
gp350/220 envelope Ag. In contrast, binding of gp350+ particles, released by
EBV-infected cells, to B cell lines or autologous primary B lymphocytes also
promoted specific Ab-dependent NK cell degranulation and TNF-α production but
induced minimal IFN-γ secretion. In that case, target cell damage appeared
marginal compared with the effect of a control anti-CD20 Ab (rituximab) at
concentrations that triggered similar NK cell activation, indicating that
cell-associated gp350+ particles may divert the cytolytic machinery, impairing
its direct action on the plasma membrane. These observations support that
Ab-dependent NK cell activation plays an important role in the control of EBV,
enhancing NK cell effector functions against infected B cells in the lytic
cycle. In contrast, the data reveal that gp350+ particles bound to bystander B
cells trigger Ab-dependent NK cell degranulation and TNF-α but not cytotoxicity
or IFN-γ production, potentially favoring the progression of viral infection.
Reference:
Product link:
Specialized antibody services, Conjugation:
http://www.immunostep.com/content/30-specialized-antibody-services
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