An article published this year in “ODONTOLOGY” using our FITC-conjugated
Annexin-V and 7-AAD, by our customers from Cellular Therapy and Hematopoietic Transplant
Unit, Haematology Department, Virgen de la Arrixaca Clinical University
Hospital, IMIB, University of Murcia, Spain, in the analysis of Biological effects
of silk fibroin 3D scaffolds on stem cells from human exfoliated deciduous
teeth (SHEDs). Congrats and Thanks.
Summary:
The aim is to investigate in vitro
biological effects of silk fibroin 3D scaffolds on stem cells from human
exfoliated deciduous teeth (SHEDs) in terms of proliferation, morphological
appearance, cell viability, and expression of mesenchymal stem cell markers.
Silk fibroin 3D scaffolding materials may represent promising suitable
scaffolds for their application in regenerative endodontic therapy approaches.
SHEDs were cultured in silk fibroin 3D scaffolds. Then, cell numbers were
counted and the Alamar blue colorimetric assay was used to analyse cell
proliferation after 24, 48, 72, and 168 h of culture. The morphological
features of SHEDs cultured on silk fibroin scaffolds were evaluated by scanning
electron microscopy (SEM). Finally, cell viability and the expression of
mesenchymal stem cell markers were analysed by flow cytometry. One-way analysis
of variance (ANOVA) followed by a Bonferroni post-test was performed (P <
0.05). At 24 and 48 h of culture, SHED proliferation on scaffolds was modest
compared to the control although still significant (p < 0.05). However, cell
proliferation progressively increased from 72 to 168 h compared with the
control (p < 0.001; p < 0.01). In addition, flow cytometry analysis
showed that the culture of SHEDs on silk fibroin scaffolds did not
significantly alter the level of expression of the mesenchymal markers CD73,
CD90, or CD105 up to 168 h; in addition, cell viability in silk fibroin was
similar to than obtained in plastic. Moreover, SEM studies revealed a suitable
degree of proliferation, cell spreading, and attachment, especially after 168 h
of culture. The findings from the current study suggest that silk fibroin 3D
scaffolds had a favourable effect on the biological responses of SHEDs. Further
in vivo investigations are required to confirm these results.
Reference:
Product link:
FITC Annexin V Apoptosis Detection
Kit with 7-AAD: http://www.immunostep.com/apoptosis-tools/3741-anxvkf-100t.html
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