An article published this year in “INVESTIGATIONAL NEW DRUGS” using our RNase solution and annexin V-fluorescein
isothiocyanate (FITC)/PI double staining kit, by our customers from CNC.IBILI University of
Coimbra, Portugal, in the analysis of how Urolithins impair cell proliferation,
arrest the cell cycle and induce apoptosis in UMUC3 bladder cancer cells.
Congrats and Thanks.
Summary:
Ellagitannins have been gaining
attention as potential anticancer molecules. However, the low bioavailability
of ellagitannins and their extensive metabolization in the gastrointestinal
tract into ellagic acid and urolithins suggest that the health benefits of
consuming ellagitannins rely on the direct effects of their metabolites.
Recently, chemopreventive and chemotherapeutic activities were ascribed to
urolithins. Nonetheless, there is still a need to screen and evaluate the
selectivity of these molecules and to elucidate their cellular mechanisms of
action. Therefore, this work focused on the antiproliferative effects of urolithins
A, B and C and ellagic acid on different human tumor cell lines. The evaluation
of cell viability and the determination of the half-maximal inhibitory
concentrations indicated that the sensitivity to the studied urolithins varied
markedly between the different cell lines, with the bladder cancer cells
(UMUC3) being the most susceptible. In UMUC3 cells, urolithin A was the most
active molecule, promoting cell cycle arrest at the G2/M checkpoint, increasing
apoptotic cell death and inhibiting PI3K/Akt and MAPK signaling. Overall, the
present study emphasizes the chemopreventive/chemotherapeutic potential of
urolithins, highlighting the stronger effects of urolithin A and its potential
to target transitional bladder cancer cells.
Reference:
Product link:
CELL CYCLE ANALYSIS (PI / RNASE): http://www.immunostep.com/solutions-buffers-chemicals/3870-pi-rnase-solution.html
FITC Annexin V Apoptosis Detection
Kit with PI: http://www.immunostep.com/apoptosis-tools/3727-anxvkf-100t.html
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