An article published this year in “BIOCHIMIE” using our PI/RNASE
Kit Solution and Annexin
V-FITC Apoptosis Detection kit, by our customers from Laboratório de
Oncologia Experimental e Hemopatias, Departamento de Análises Clínicas,
Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil, in the
analysis of Cytotoxic effect of a novel naphthylchalcone against multiple
cancer cells focusing on hematologic malignancies. Congrats and Thanks.
Summary:
Chalcones are natural compounds
described in the literature by its several properties including cytotoxic
activity against several tumor types. Considering that the search for new
chemotherapeutic agents is still necessary, the aim of this study was to
investigate the cytotoxic mechanisms involved in cell death induced by a
synthetic chalcone (A23) on different tumor cells. Chalcone A23 reduced the
cell viability of twelve tumor cell lines in a concentration and time dependent
manner and it was more cytotoxic against acute leukemia cells. Interestingly,
the compound was non cytotoxic to normal cells and non-hemolytic to normal red
blood cells. Chalcone A23 decreased the expression of cell proliferation marker
KI-67 and blocked the G2/M phase in both K562 and Jurkat cell lines. Cells
treated with A23 showed morphological features suggestive of apoptosis, the
“latter pattern” in agarose gel, the externalization of phosphatidylserine and
caspase-3 and PARP cleavage. Chalcone A23 significantly reduced the
mitochondrial membrane potential, decreased the expression of anti-apoptotic
proteins Bcl-2 and survivin and increased the expression of pro-apoptotic
protein Bax, confirming the involvement of the intrinsic pathway. The increased
mitochondrial permeability resulted in the release of AIF, cytochrome c and
endonuclease G from the mitochondria to the cytosol. In addition, chalcone A23
increased the expression of FasR and induced Bid cleavage, showing the
involvement of the extrinsic pathway. Finally, chalcone A23 seems to have a
synergic effect with the chemotherapy drugs cytarabine and vincristine. These
results suggest that A23 is an interesting compound with strong and selective
anti-tumor activity.
Reference:
Product link:
PI/RNASE Kit Solution: http://www.immunostep.com/solutions-buffers-chemicals/3870-pi-rnase-solution.html
Annexin V-FITC Apoptosis Detection
kit: http://www.immunostep.com/apoptosis-tools/3727-anxvkf-100t.html
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