An article published this year in “Cell
Death and Disease” using our “Apoptosis
Detection Kit PE”, by our customers from the Instituto de Salud Carlos III
(ISCIII), Majadahonda, Madrid, Spain, in
the analysis of how Altered marginal zone and innate-like B cells in aged
senescence-accelerated SAMP8 mice with defective IgG1 responses. Congrats and
Thanks.
Summay:
Aging has a
strong impact on the activity of the immune system, enhancing susceptibility to
pathogens and provoking a predominant pre-inflammatory status, whereas
dampening responses to vaccines in humans and mice. Here, we demonstrate a loss
of marginal zone B lymphocytes (MZ, CD19+ CD45R+ CD21++CD23lo) and a decrease
of naive B cells (CD19+ IgD+ ), whereas there is an enhancement of a CD19+
CD45Rlo innate-like B cell population (B1REL) and the so-called aged B cell
compartment (ABC, CD45R+ CD21loCD23loCD5−CD11b−) in aged senescence-accelerated
(SAMP8) mice but not in aged senescence-resistant (SAMR1) mice. These changes
in aged SAMP8 mice were associated with lower IgG isotype levels, displaying
low variable gene usage repertoires of the immunoglobulin heavy chain (VH)
diversity, with a diminution on IgG1-memory B cells (CD11b−Gr1−CD138−
IgM−IgD−CD19+ CD38+ IgG1+ ), an increase in T follicular helper (TFH, CD4+
CXCR5+ PD1+ ) cell numbers, and an altered MOMA-1 (metallophilic macrophages)
band in primary follicles. LPS-mediated IgG1 responses were impaired in the
B1REL and ABC cell compartments, both in vitro and in vivo. These data
demonstrate the prominent changes to different B cell populations and in
structural follicle organization that occur upon aging in SAMP8 mice. These
novel results raise new questions regarding the importance of the cellular
distribution in the B cell layers, and their effector functions needed to mount
a coordinated and effective humoral response.
Reference:
Product link:
Apoptosis Detection Kit PE
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