An article published this year in “Biomedicine &
Pharmacotherapy”
using our “Apoptosis Detection Kit FITC”, by our customers from Chandigarh, India., in the analysis of how Autophagy as a
potential therapeutic target during epithelial to mesenchymal transition in
renal cell carcinoma: An in vitro study. Congrats and Thanks.
Summay:
Cancer
progression toward invasive and metastatic disease is aided by reactivation of epithelial–mesenchymal
transition (EMT),
involving transdifferentiation of epithelial cellsinto mesenchymal phenotype.
This leads to increased migratory and stem cell-like features in the cells.
These EMT cells are more resistant to chemotherapy and it is hypothesized that the phenomenon of autophagy induces
resistance, providing a survival strategy for cells.
In the present study, we induced EMT-like
phenotype in renal carcinoma cells and
identified corresponding higher autophagy flux in these cells. The EMT
transformed cells may be a representative of the resistant cancer stem
cell(CSC)-like phenotype. Autophagy was identified as a potential mechanism of
cell survival in these cells thus implying that autophagy inhibition can lead
to enhanced cell death. We also observed that tumor cellsespecially
EMT transformed cells, have been ‘primed’ to undergo autophagy by mTOR
inhibition. We observed that combined use of autophagy inhibitor and temsirolimus (TEM) improved antitumor activity against
RCC in EMT transformed metastatic cells. One of the approaches for inhibiting
autophagy was the use of lysosomotropic anti-malarial drug, chloroquine (CQ) and we explored the therapeutic potential
of combination of CQ and the mTOR inhibitor, TEM. EMT transformed cells showed
increased cell cytotoxicity when
autophagy was impaired by addition CQ with TEM. This led us to conclude that
inhibition of autophagy with the current therapeutic regimen could be useful in
targeting the EMT transformed cells along with the bulk tumor cells in RCC.
Reference:
Product link:
Apoptosis Detection Kit FITC
http://www.immunostep.com/apoptosis/3727-anxvkf-100t.html
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