An article published this year in the “Wiley Online Library, International Journal of Laboratory Hematology” using one of our antibodies “APC anti-mouse CD3e clone 145-2C11”, by our customers from Città della Salute e della Scienza di Torino, Italy, in the analysis of Relevance of sample preparation for flow cytometry. Congrats and Thanks.
Flow cytometry is a useful tool for diagnosis and minimal residual disease (MRD) study of hematological diseases. Standard sample preparation protocols are characterized by stain-lyse-wash (SLW). To prevent nonspecific bindings and achieve high sensitivity in MRD studies, lyse-wash-stain-wash (LWSW) is required. To our knowledge, no comparison between the two methods has been performed.
Bland-Altman analysis of percentages of cells positive for 104 antibodies in samples simultaneously prepared with LWSW and SLW methods
APC anti-mouse CD3e clone 145-2C11