An article published this year in “Front.
Cell. Infect. Microbiol”
using our our “Apoptosis Detection Kit FITC”, by our customers from SaBio, Instituto de
Investigación en Recursos Cinegéticos, Ciudad Real, Spain, in the analysis of how Vaccinomics Approach to
the Identification of Candidate Protective Antigens for the Control of Tick
Vector Infestations and Anaplasma phagocytophilum Infection. Congrats
and Thanks.
Summay:
Anaplasma phagocytophilum is an emerging tick-borne pathogen causing human
granulocytic anaplasmosis (HGA), tick-borne fever (TBF) in small ruminants, and
other forms of anaplasmosis in different domestic and wild animals. The main
vectors of this pathogen are Ixodes tick species,
particularly I. scapularis in the United States and I. ricinus in Europe.
One of the main limitations for the development of effective vaccines for the
prevention and control of A. phagocytophiluminfection and
transmission is the identification of effective tick protective antigens. The
objective of this study was to apply a vaccinomics approach to I. scapularis-A. phagocytophilum interactions for the identification and
characterization of candidate tick protective antigens for the control of
vector infestations and A. phagocytophilum infection.
The vaccinomics pipeline included the use of quantitative transcriptomics and
proteomics data from uninfected and A. phagocytophilum-infected I. scapularis ticks for the
selection of candidate protective antigens based on the variation in tick mRNA
and protein levels in response to infection, their putative biological
function, and the effect of antibodies against these proteins on tick cell
apoptosis and pathogen infection. The characterization of selected candidate
tick protective antigens included the identification and characterization of I. ricinus homologs,
functional characterization by different methodologies including RNA
interference, immunofluorescence, gene expression profiling, and artificial
tick feeding on rabbit antibodies against the recombinant antigens to select
the candidates for vaccination trials. The vaccinomics pipeline developed in
this study resulted in the identification of two candidate tick protective
antigens that could be selected for future vaccination trials. The results
showed that I. scapularis lipocalin (ISCW005600) and lectin pathway inhibitor
(AAY66632) and I. ricinus homologs constitute candidate protective antigens for
the control of vector infestations and A. phagocytophilum infection.
Both antigens are involved in the tick evasion of host defense response and
pathogen infection and transmission, but targeting different immune response
pathways. The vaccinomics pipeline proposed here could be used to continue the
identification and characterization of candidate tick protective antigens for
the development of effective vaccines for the prevention and control of HGA,
TBF, and other forms of anaplasmosis caused by A. phagocytophilum.
Reference:
Product link:
Apoptosis Detection Kit FITC
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