An article published
this year in “Proteomes”
using some of our products, “FITC
Mouse Anti-Human CD14, APC
Mouse Anti-Human CD19 and OC-515 Mouse Anti-Human CD45”, by
some of our customers present some Proteomics Units from Universities of Navarra,
Valencia, Madrid, Basque Country and Salamanca, in the analysis of In-Depth
Proteomic Characterization of Classical and Non-Classical Monocyte Subsets Antitumor
Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two
Monocyte Isolation Methods. Congrats and Thanks.
Summay:
Monocytes are bone marrow-derived
leukocytes that are part of the innate immune system. Monocytes are divided
into three subsets: classical, intermediate and non-classical, which can be
differentiated by their expression of some surface antigens, mainly CD14 and
CD16.
These cells are key players in
the inflammation process underlying the mechanism of many diseases. Thus, the
molecular characterization of these cells may provide very useful information for
understanding their biology in health and disease. We performed a multicentric proteomic
study with pure classical and non-classical populations derived from 12 healthy
donors. The robust workflow used provided reproducible results among the five
participating laboratories. Over 5000 proteins were identified, and about half
of them were quantified using a spectral counting approach.
The results represent the protein
abundance catalogue of pure classical and enriched non-classical blood
peripheral monocytes, and could serve as a reference dataset of the healthy
population. The functional analysis of the differences between cell subsets
supports the consensus roles assigned to human monocytes.
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Protein abundance correlation between purified
monocyte populations and public datasets obtained from PaxDB. Protein abundances determined in
the present work (y-axis) are plotted against public datasets (x-axis). CD14 (classical) and CD16
(non-classical), are shown in left and right panels, respectively. Complete monocyte population and liver
datasets are shown in the top panels and bottom panels, respectively.
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