Monday, 29 May 2017

FITC Annexin V Apoptosis Detection Kit with PI.

An article published this year in “NANOMATERIALS” using our Annexin V–fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) double staining by our customers from Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Italy, in the analysis of Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells. Congrats and Thanks.


Infections of orthopedic implants are associated with high morbidity. The emergence of antibiotic resistant strains and the tendency of microbes to form biofilms on orthopedic devices prompt the individuation of novel antimicrobial agents. Silver nanoparticles represent an interesting alternative, but their effects on bone cells need to be clarified. We focused on osteoblast-like cells and on bone marrow-mesenchymal stem cells and found that these cells are rather resistant to the cytotoxic effects of silver nanoparticles, with a half maximal inhibitory concentration around 25 µg/mL as detected by MTT assay. Within a month of treatment, osteoblast-like cells adapt to the presence of the nanoparticles by upregulating hsp70 as shown by western blot. Hsp70 overexpression correlates with the restoration of normal cell proliferation. No alterations in the extent and time requirements were detected in mesenchymal stem cell induced to differentiate in osteoblasts in the presence of silver nanoparticles. Because the concentrations of silver nanoparticles which show antimicrobial activity are lower than those exerting toxic effects on bone-forming cells in vitro, we suggest that silver nanoparticles might represent a challenging tool to fight infections in orthopedic implants.


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