An article published this year in “STEM CELLS INTERNATIONAL” using our Annexin
V–fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) double
staining, by our customers from Department of Endodontics, Faculty of
Medicine and Dentistry, Catholic University of Valencia “San Vicente Mártir”,
Valencia, Spain, in the analysis of Cellular Responses in Human Dental Pulp
Stem Cells Treated with Three Endodontic Materials. Congrats and Thanks.
Summary:
Human dental pulp stem cells
(HDPSCs) are of special relevance in future regenerative dental therapies.
Characterizing cytotoxicity and genotoxicity produced by endodontic materials
is required to evaluate the potential for regeneration of injured tissues in
future strategies combining regenerative and root canal therapies. This study
explores the cytotoxicity and genotoxicity mediated by oxidative stress of three
endodontic materials that are widely used on HDPSCs: a mineral trioxide
aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an
MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were
assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of
antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by
quantifying the expression levels of DNA damage sensors such as ATM and RAD53
genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate
that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in
HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and
genotoxic material for HDPSCs at longer times in preincubated cell culture
medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and
MTA-Fillapex at all times assayed.
Reference:
Product link:
FITC Annexin V Apoptosis Detection Kit with PI: http://www.immunostep.com/apoptosis-tools/3727-anxvkf-100t.html
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