Friday 29 December 2017

An article published in “Injury, International Journal of the Care of the Injured” using our PerCP-Cyanine5.5 Mouse Anti-Human CD45, clone D3/9 by our customers from the  National Institute of Traumatology and Orthopedics, Rio de Janeiro, Brazil, in the analysis of how Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment. Congrats and Thanks.


Summary:
Femoral shaft fractures generally occur in young adults following a high-energy trauma and are prone to delayed union/non-union. Novel therapies to stimulate bone regeneration will have to mimic some of the aspects of the biology of fracture healing; however, which are these aspects is unclear. Locked intramedullary nailing is the current treatment of choice for the stabilisation of femur shaft fractures, and it is associated with accelerated healing and increased union rates. These benefits were partially attributed to the reaming procedure, which, regardless of significantly destroying the haematoma, stimulates the healing response. To better understand how reaming influences healing, we evaluated the viability of the nucleated cell fraction and the frequency of CD146+skeletal progenitors, which contain multipotent cells, in the post-reaming haematoma. We also screened the concentrations of inflammatory mediators and growth factors in the fracture site after reaming compared with those in the original haematoma.

 

(A, B) Representative flow cytometry analysis of the CD45/CD31/CD146+ skeletal cell population in a representative pre- and post-reaming haematoma. Analyses were performed in cells gated subsequently in R1, R2, R3, and R4.

Reference:


Product link:

PerCP-Cyanine5.5 Mouse Anti-Human CD45, clone D3/9

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