Friday 28 July 2017

An article published this year in “Analytical and Bioanalytical Chemistry using our “Polyclonal Antibody Service”, by our customers from Faculty of ChemistryComplutense University of Madrid, Humanitas Clinical and Research Center, Rozzano, Italy, in the analysis of how MicroRNA-26a/cyclin-dependent kinase 5 axis controls proliferation, apoptosis and in vivo tumor growth of diffuse large B-cell lymphoma cell lines. Congrats and Thanks.

Summay:
The assembly of a novel disposable amperometric immunosensor for the detection of the red wine spoilage yeast Brettanomyces bruxellensis is reported. The nanostructured sensing interface was prepared by first coating carbon screen printed electrodes with a gold nanoparticles-reduced graphene oxide hybrid nanomaterial, which was then modified with 3-mercaptopropionic acid to further immobilize specific antibodies for B. bruxellensis via a carbodiimide-coupling reaction. The functionalized electrode allowed the amperometric detection of B. bruxellensis in buffered solutions and red wine samples in the range of 10–106 CFU/mL and 102–106 CFU/mL, with low detection limits of 8 CFU/mL and 56 CFU/mL, respectively. The electrochemical immunosensor also exhibited high reproducibility, selectivity, and storage stability.

Reference:

Product link:
Immunostep Polyclonal Antibody Service

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