An article published this year in “NANOSCALE” using our CFBlue
Annexin V Apoptosis Detection Kit with PI, by our customers from Department
of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre
(IBMCC/CSIC/USAL/IBSAL), Salamanca, Spain., in the analysis of Functional
insights into the cellular response triggered by a bile-acid platinum compound
conjugated to biocompatible ferric nanoparticles using quantitative proteomic
approaches.. Congrats and Thanks.
Summary:
At present, bioferrofluids are
employed as powerful multifunctional tools for biomedical applications such as
drug delivery, among others. The present study explores the cellular response
evoked when bile-acid platinum derivatives are conjugated with bioferrofluids
by testing the biological activity in osteosarcoma (MG-63) and T-cell leukemia
(Jurkat) cells. The aim of this work is to evaluate the biocompatibility of a
bile-acid platinum derivative conjugated with multi-functional polymer coated
bioferrofluids by observing the effects on the protein expression profiles and
in intracellular pathways of nanoparticle-stimulated cells. To this end, a mass
spectrometry-based approach termed SILAC has been applied to determine in a
high-throughput manner the key proteins involved in the cellular response
process (including specific quantitatively identified proteins related to the
vesicular transport, cellular structure, cell cycle, biosynthetic process,
apoptosis and regulation of the cell cycle). Finally, biocompatibility was
evaluated and validated by conventional strategies also (such as flow
cytometry, MTT, etc.).
Reference:
Product link:
CFBlue Annexin V Apoptosis
Detection Kit with PI: http://www.immunostep.com/apoptosis-tools/3735-anxvkcfb-100t.html
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